ArticlePloS one2015
A Nematode Calreticulin, Rs-CRT, Is a Key Effector in Reproduction and Pathogenicity of Radopholus similis.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 34 citations in OpenAlex.
- A novel calreticulin of Psoroptes ovis regulated keratinocyte function resulting in host skin barrier dysfunction: implications for involvement in the pathogenesis of psoroptic mange.Parasites & vectors · 2025Article
- Advances in Migratory Plant Endoparasitic Nematode Effectors.International journal of molecular sciences · 2024Review
- Transgenic East African Highland Banana Plants Are Protected againstInternational journal of molecular sciences · 2023Article
- The many faces of parasite calreticulin.Frontiers in immunology · 2023Review
- Nematicidal Property of Clindamycin and 5-hydroxy-2-methyl Furfural (HMF) from the Banana EndophyteIndian journal of microbiology · 2022Article
- Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis.PLoS pathogens · 2021Article
- A Venom Allergen-Like Protein, RsVAP, the First Discovered Effector Protein ofInternational journal of molecular sciences · 2021Article
- Current Insights into Migratory Endoparasitism: Deciphering the Biology, Parasitism Mechanisms, and Management Strategies of Key Migratory Endoparasitic Phytonematodes.Plants (Basel, Switzerland) · 2020Review
- HaCRT1 ofFrontiers in plant science · 2020Article
- Identification and Characterization of a Fatty Acid- and Retinoid-Binding Protein Gene (International journal of molecular sciences · 2019Article
- Life-stage specific transcriptomes of a migratory endoparasitic plant nematode, Radopholus similis elucidate a different parasitic and life strategy of plant parasitic nematodes.Scientific reports · 2019Article
- Article
- The genome of the migratory nematode, Radopholus similis, reveals signatures of close association to the sedentary cyst nematodes.PloS one · 2019Article
- Rational design of biosafe crop resistance to a range of nematodes using RNA interference.Plant biotechnology journal · 2018Article
- Transgenic Nicotiana benthamiana plants expressing a hairpin RNAi construct of a nematode Rs-cps gene exhibit enhanced resistance to Radopholus similis.Scientific reports · 2017Article
- Genetically engineered bananas resistant to Xanthomonas wilt disease and nematodes.Food and energy security · 2017Review
- The Distribution of Lectins across the Phylum Nematoda: A Genome-Wide Search.International journal of molecular sciences · 2017Article
- Smart Parasitic Nematodes Use Multifaceted Strategies to Parasitize Plants.Frontiers in plant science · 2017Review
- The cathepsin S cysteine proteinase of the burrowing nematode Radopholus similis is essential for the reproduction and invasion.Cell & bioscience · 2016Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Radopholus similis is a migratory plant-parasitic nematode that causes severe damage to many agricultural and horticultural crops. Calreticulin (CRT) is a Ca2+-binding multifunctional protein that plays key roles in the parasitism, immune evasion, reproduction and pathogenesis of many animal parasites and plant nematodes. Therefore, CRT is a promising target for controlling R. similis. In this study, we obtained the full-length sequence of the CRT gene from R. similis (Rs-crt), which is 1,527-bp long and includes a 1,206-bp ORF that encodes 401 amino acids. Rs-CRT and Mi-CRT from Meloidogyne incognita showed the highest similarity and were grouped on the same branch of the phylogenetic tree. Rs-crt is a multi-copy gene that is expressed in the oesophageal glands and gonads of females, the gonads of males, the intestines of juveniles and the eggs of R. similis. The highest Rs-crt expression was detected in females, followed by juveniles, eggs and males. The reproductive capability and pathogenicity of R. similis were significantly reduced after treatment with Rs-crt dsRNA for 36 h. Using plant-mediated RNAi, we confirmed that Rs-crt expression was significantly inhibited in the nematodes, and resistance to R. similis was significantly improved in transgenic tomato plants. Plant-mediated RNAi-induced silencing of Rs-crt could be effectively transmitted to the F2 generation of R. similis; however, the silencing effect of Rs-crt induced by in vitro RNAi was no longer detectable in F1 and F2 nematodes. Thus, Rs-crt is essential for the reproduction and pathogenicity of R. similis.
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