ArticleIranian journal of public health2014
Genotyping and Phylogenetic Analysis of Fasciola Spp. Isolated from Sheep and Cattle Using PCR-RFLP in Ardabil Province, Northwestern Iran.
Article in Iranian journal of public health, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
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- Evaluation of Semi-Nested PCR Compared with Indirect-ELISA to Diagnose Human Fasciolosis.Iranian journal of public health · 2022Article
- Molecular Identification ofJournal of parasitology research · 2022Article
- Identification and Genetic Characterization ofInfection and drug resistance · 2022Article
- Molecular Characterization ofIranian journal of public health · 2020Article
- Identification and Phylogenetic Classification ofIranian journal of public health · 2019Article
- High-Resolution Melting Analysis as an Appropriate Method to Differentiate betweenIranian journal of public health · 2019Article
- Molecular Evidence of Human Fasciolosis Due toIranian journal of public health · 2018Article
- Identification ofJournal of parasitology research · 2018Article
- Molecular and Morphometrical Characterization ofIranian journal of public health · 2017Article
- Molecular Characterization of AnimalIranian journal of public health · 2016Article
- Molecular characterization ofVeterinary world · 2016Article
- The Larval Stages ofIranian journal of parasitologyArticle
- Molecular Characterization of Liver Fluke Isolated from Sheep, Goat and Cattle in Sulaymaniyah, Iraq.Iranian journal of parasitologyArticle
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe aim of this study was to detect the genotype of Fasciola spp. in Meshkin-Shahr, Ardabil Province, northwestern Iran in different hosts using PCR-RFLP.
methodsThe parasite hosts included cattle, and sheep. Overall, 70 adult flukes from livers of slaughtered animals were collected from the abattoirs of aforementioned area. The included 35 samples from infected sheep and 35 samples from 35 infected cattle. PCR-RFLP and sequence analysis of the first nuclear ribosomal internal transcribed spacer (ITS 1) region from Fasciola species were used to conduct the study.
resultsThe fragment of approximately 700bp in all of the Fasciola samples was amplified. PCR products of ITS 1 were subjected for digestion by restriction enzyme. RsaI restriction enzyme was selected for RFLP method that caused the separation specifically of Fasciola species. Amplicons with the sequences of F. hepatica had a pattern of about 360, 100, and 60 bp band size, whereas F. gigantica worms had a profile of 360, 170, and 60 bp in size, respectively. Results based on PCR-RFLP analysis were confirmed by sequence analysis of representative ITS 1 amplicons. No hybrid forms were detected in the present study. All sheep were infected with F. hepatica but cattle were infected with both species.
conclusionBoth species of Fasciola are present in Ardabil. The method described here can be valuable for identification of Fasciola species in endemic parts for fasciolosis, regions with intermediate species and in that overlapping distribution area.
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