ArticleEmerging microbes & infections2015
Evaluation of zebrafish as a model to study the pathogenesis of the opportunistic pathogen Cronobacter turicensis.
Article in Emerging microbes & infections, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 26 citations in OpenAlex.
- Phylosymbiosis and functional redundancy in the Drosophila (Diptera: Drosophilidae) gut microbiome and its implications for host fitness.Journal of insect science (Online) · 2026Article
- Diving into drug-screening: zebrafish embryos as an in vivo platform for antimicrobial drug discovery and assessment.FEMS microbiology reviews · 2024Review
- Influence of Methylene Blue or Dimethyl Sulfoxide on Larval Zebrafish Development and Behavior.Zebrafish · 2023Article
- Characterization ofMicroorganisms · 2022Article
- Article
- Zebrafish Bacterial Infection Assay to Study Host-Pathogen Interactions.Bio-protocol · 2020Article
- Zebrafish as an alternative animal model in human and animal vaccination research.Laboratory animal research · 2020Review
- Analysis of the Molecular Diversity AmongFrontiers in microbiology · 2020Article
- Internal Versus External Pressures: Effect of Housing Systems on the Zebrafish Microbiome.Zebrafish · 2019Article
- Zebrafish Granulocyte Colony-Stimulating Factor Receptor Maintains Neutrophil Number and Function throughout the Life Span.Infection and immunity · 2019Article
- Effects of the Antimicrobial Peptide LL-37 and Innate Effector Mechanisms in Colistin-ResistantFrontiers in microbiology · 2019Article
- Genomic characterization of malonate positiveGut pathogens · 2018Article
- Comparative Genomic Characterization of the Highly Persistent and Potentially VirulentFrontiers in microbiology · 2017Article
- The DSF type quorum sensing signalling system RpfF/R regulates diverse phenotypes in the opportunistic pathogen Cronobacter.Scientific reports · 2016Article
- A Zebrafish Model forFrontiers in microbiology · 2016Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacteria belonging to the genus Cronobacter spp. have been recognized as causative agents of life-threatening systemic infections, primarily in premature, low-birth weight and/or immune-compromised neonates. Knowledge remains scarce regarding the underlying molecular mechanisms of disease development. In this study, we evaluated the use of a zebrafish model to study the pathogenesis of Cronobacter turicensis LMG 23827(T), a clinical isolate responsible for two fatal sepsis cases in neonates. Here, the microinjection of approximately 50 colony forming units (CFUs) into the yolk sac resulted in the rapid multiplication of bacteria and dissemination into the blood stream at 24 h post infection (hpi), followed by the development of a severe bacteremia and larval death within 3 days. In contrast, the innate immune response of the embryos was sufficiently developed to control infection after the intravenous injection of up to 10(4) CFUs of bacteria. Infection studies using an isogenic mutant devoid of surviving and replicating in human macrophages (ΔfkpA) showed that this strain was highly attenuated in its ability to kill the larvae. In addition, the suitability of the zebrafish model system to study the effectiveness of antibiotics to treat Cronobacter infections in zebrafish embryos was examined. Our data indicate that the zebrafish model represents an excellent vertebrate model to study virulence-related aspects of this opportunistic pathogen in vivo.
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