ArticleThe Journal of biological chemistry2015
Src Kinase Is the Connecting Player between Protein Kinase A (PKA) Activation and Hyperpolarization through SLO3 Potassium Channel Regulation in Mouse Sperm.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 43 citations in OpenAlex.
- A Scalable Flow Cytometry Assay to Evaluate CatSper-mediated CaAndrology · 2026Article
- JAGGED1 modulates bull spermatozoa acrosome reaction and fertilization.Reproduction & fertility · 2026Article
- Dual role of valosin-containing protein (VCP/p97) in mouse sperm during capacitation.Reproduction (Cambridge, England) · 2024Article
- High-throughput screening method for discovering CatSper inhibitors using membrane depolarization caused by external calcium chelation and fluorescent cell barcoding.Frontiers in cell and developmental biology · 2023Article
- A Review on the Role of Bicarbonate and Proton Transporters during Sperm Capacitation in Mammals.International journal of molecular sciences · 2022Review
- The early molecular events leading to COFILIN phosphorylation during mouse sperm capacitation are essential for acrosomal exocytosis.The Journal of biological chemistry · 2022Article
- A Minimal Model Shows that a Positive Feedback Loop Between sNHE and SLO3 can Control Mouse Sperm Capacitation.Frontiers in cell and developmental biology · 2022Article
- Mathematical model reveals that heterogeneity in the number of ion transporters regulates the fraction of mouse sperm capacitation.PloS one · 2021Article
- Male Decapacitation Factor SPINK3 Blocks Membrane Hyperpolarization and Calcium Entry in Mouse Sperm.Frontiers in cell and developmental biology · 2020Article
- Src family kinases-mediated negative regulation of sperm acrosome reaction in chickens (Gallus gallus domesticus).PloS one · 2020Article
- Redox Regulation and Oxidative Stress: The Particular Case of the Stallion Spermatozoa.Antioxidants (Basel, Switzerland) · 2019Review
- Inhibition of non-receptor tyrosine kinase Src induces phosphoserine 256-independent aquaporin-2 membrane accumulation.The Journal of physiology · 2019Article
- Sperm capacitation is associated with phosphorylation of the testis-specific radial spoke protein Rsph6a†.Biology of reproduction · 2019Article
- Membrane Potential Assessment by Fluorimetry as a Predictor Tool of Human Sperm Fertilizing Capacity.Frontiers in cell and developmental biology · 2019Article
- Super-resolution imaging of live sperm reveals dynamic changes of the actin cytoskeleton during acrosomal exocytosis.Journal of cell science · 2018Article
- Lysine acetylation modulates mouse sperm capacitation.Scientific reports · 2018Article
- CFTR/ENaC-dependent regulation of membrane potential during human sperm capacitation is initiated by bicarbonate uptake through NBC.The Journal of biological chemistry · 2018Article
- Disruption of protein kinase A localization induces acrosomal exocytosis in capacitated mouse sperm.The Journal of biological chemistry · 2018Article
- Molecular Basis of Human Sperm Capacitation.Frontiers in cell and developmental biology · 2018Review
- Essential Role of CFTR in PKA-Dependent Phosphorylation, Alkalinization, and Hyperpolarization During Human Sperm Capacitation.Journal of cellular physiology · 2017Article
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 2 countries.
Funding
Abstract
Plasma membrane hyperpolarization is crucial for mammalian sperm to acquire acrosomal responsiveness during capacitation. Among the signaling events leading to mammalian sperm capacitation, the immediate activation of protein kinase A plays a pivotal role, promoting the subsequent stimulation of protein tyrosine phosphorylation that associates with fertilizing capacity. We have shown previously that mice deficient in the tyrosine kinase cSrc are infertile and exhibit improper cauda epididymis development. It is therefore not clear whether lack of sperm functionality is due to problems in epididymal maturation or to the absence of cSrc in sperm. To further address this problem, we investigated the kinetics of cSrc activation using anti-Tyr(P)-416-cSrc antibodies that only recognize active cSrc. Our results provide evidence that cSrc is activated downstream of PKA and that inhibition of its activity blocks the capacitation-induced hyperpolarization of the sperm plasma membrane without blocking the increase in tyrosine phosphorylation that accompanies capacitation. In addition, we show that cSrc inhibition also blocks the agonist-induced acrosome reaction and that this inhibition is overcome by pharmacological hyperpolarization. Considering that capacitation-induced hyperpolarization is mediated by SLO3, we evaluated the action of cSrc inhibitors on the heterologously expressed SLO3 channel. Our results indicate that, similar to SLO1 K(+) channels, cSrc blockers significantly decreased SLO3-mediated currents. Together, these results are consistent with findings showing that hyperpolarization of the sperm plasma membrane is necessary and sufficient to prepare the sperm for the acrosome reaction and suggest that changes in sperm membrane potential are mediated by cSrc activation.
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