Evidence map›Paper›PMID 26060080›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2015

Coupling Binding to Catalysis: Using Yeast Cell Surface Display to Select Enzymatic Activities.

Keya Zhang, Karan Bhuripanyo, Yiyang Wang, Jun Yin

Abstract read
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Keya ZhangDepartment of Chemistry, University of Chicago, 929 E57th Street, Chicago, IL, 60637, USA.
Karan Bhuripanyo
Yiyang Wang
Jun Yin

Funding

Orthogonal Ubiquitin Transfer to Profile E3 Substrate Specificity.R01GM104498 · NIGMS · UNIVERSITY OF CHICAGO · PI KIYOKAWA, HIROAKI, YIN, JUN · 2013 to 2021
$3.2M
NIGMS NIH HHS 1R01GM104498NIGMS NIH HHS R01 GM104498
6 · The paper itself

Abstract

We find yeast cell surface display can be used to engineer enzymes by selecting the enzyme library for high affinity binding to reaction intermediates. Here we cover key steps of enzyme engineering on the yeast cell surface including library design, construction, and selection based on magnetic and fluorescence-activated cell sorting.

Indexed as

CatalysisCell SeparationCell Surface Display TechniquesEnzymesFlow CytometryPeptide LibraryProtein BindingProtein EngineeringSaccharomyces cerevisiaeSubstrate SpecificityEnzymesPeptide Library

Identifiers

PMID26060080
PMCPMC4648535

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.