Evidence map›Paper›PMID 26060074›Full record

ReviewMethods in molecular biology (Clifton, N.J.)2015

Applications of Yeast Surface Display for Protein Engineering.

Gerald M Cherf, Jennifer R Cochran

Abstract readReview
In one paragraph

Review in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 135 papers.

0numbers the graph read from it
0cells of the map it votes in
135citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

135 citing papers in PubMed.

  1. EngineeringMicroorganisms · 2026
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  6. Differential Processing of aMicroorganisms · 2026
    Article
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  8. Discovery and functional characterization of endoglucanases fromApplied and environmental microbiology · 2026
    Article
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  15. Review
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  19. Design of a water-soluble CD20 antigen with computational epitope scaffolding.Protein science : a publication of the Protein Society · 2025
    Article
  20. Article

75 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Gerald M CherfDepartment of Bioengineering, Stanford University, Stanford, CA, 94305, USA.
Jennifer R Cochran

Funding

Engineering a multispecific cell receptor antagonist to treat metastatic cancerF31CA186478 · NCI · STANFORD UNIVERSITY · PI CHERF, GERALD MAXWELL · 2014 to 2017
$146k
NCI NIH HHS F31 CA186478NCI NIH HHS F31CA186478
6 · The paper itself

Abstract

The method of displaying recombinant proteins on the surface of Saccharomyces cerevisiae via genetic fusion to an abundant cell wall protein, a technology known as yeast surface display, or simply, yeast display, has become a valuable protein engineering tool for a broad spectrum of biotechnology and biomedical applications. This review focuses on the use of yeast display for engineering protein affinity, stability, and enzymatic activity. Strategies and examples for each protein engineering goal are discussed. Additional applications of yeast display are also briefly presented, including protein epitope mapping, identification of protein-protein interactions, and uses of displayed proteins in industry and medicine.

Indexed as

Cell Surface Display TechniquesEpitope MappingProtein BindingProtein EngineeringProtein StabilityRecombinant ProteinsSaccharomyces cerevisiaeRecombinant Proteins

Identifiers

PMID26060074
PMCPMC4544684

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.