ArticleJournal of industrial microbiology & biotechnology2015
R-acetoin accumulation and dissimilation in Klebsiella pneumoniae.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- A synergistic strategy of metabolic engineering and flocculation recycling for enhanced acetoin production inSynthetic and systems biotechnology · 2026Article
- 1,3-propanediol production by Klebsiella pneumoniae ∆dhaM∆ptsG∆glpK using glucose and glycerol as co-substrates.Microbial cell factories · 2025Article
- Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase.Microbial cell factories · 2023Review
- Metabolic Engineering of Microorganisms to Produce Pyruvate and Derived Compounds.Molecules (Basel, Switzerland) · 2023Review
- The pyruvate decarboxylase activity of IpdC is a limitation for isobutanol production by Klebsiella pneumoniae.Biotechnology for biofuels and bioproducts · 2022Article
- Blocking the 2,3-butanediol synthesis pathway of Klebsiella pneumoniae resulted in L-valine production.World journal of microbiology & biotechnology · 2022Article
- Redirection of the central metabolism of Klebsiella pneumoniae towards dihydroxyacetone production.Microbial cell factories · 2021Article
- Co-production of acetoin and succinic acid by metabolically engineered Enterobacter cloacae.Biotechnology for biofuels · 2021Article
- Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- Synthetic engineering of Corynebacterium crenatum to selectively produce acetoin or 2,3-butanediol by one step bioconversion method.Microbial cell factories · 2019Article
- Production of Chemicals by Klebsiella pneumoniae Using Bamboo Hydrolysate as Feedstock.Journal of visualized experiments : JoVE · 2017Article
- Gluconic acid production by gad mutant of Klebsiella pneumoniae.World journal of microbiology & biotechnology · 2016Article
- Metabolic engineering ofBioresources and bioprocessing · 2016Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Klebsiella pneumoniae is a 2,3-butanediol producer, and R-acetoin is an intermediate of 2,3-butanediol production. R-acetoin accumulation and dissimilation in K. pneumoniae was studied here. A budC mutant, which has lost 2,3-butanediol dehydrogenase activity, accumulated high levels of R-acetoin in culture broth. However, after glucose was exhausted, the accumulated R-acetoin could be reused by the cells as a carbon source. Acetoin dehydrogenase enzyme system, encoded by acoABCD, was responsible for R-acetoin dissimilation. acoABCD mutants lost the ability to grow on acetoin as the sole carbon source, and the acetoin accumulated could not be dissimilated. However, in the presence of another carbon source, the acetoin accumulated in broth of acoABCD mutants was converted to 2,3-butanediol. Parameters of R-acetoin production by budC mutants were optimized in batch culture. Aerobic culture and mildly acidic conditions (pH 6-6.5) favored R-acetoin accumulation. At the optimized conditions, in fed-batch fermentation, 62.3 g/L R-acetoin was produced by budC and acoABCD double mutant in 57 h culture, with an optical purity of 98.0 %, and a substrate conversion ratio of 28.7 %.
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