ArticleMagnetic resonance in medicine2016
Hyperpolarized [(13)C]ketobutyrate, a molecular analog of pyruvate with modified specificity for LDH isoforms.
Article in Magnetic resonance in medicine, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Detection of early-stage NASH using non-invasive hyperpolarizedScientific reports · 2024Article
- The metabolite alpha-ketobutyrate extends lifespan by promoting peroxisomal function in C. elegans.Nature communications · 2023Article
- Multi-band echo-planar spectroscopic imaging of hyperpolarizedMagnetic resonance in medicine · 2022Article
- Radical-free hyperpolarized MRI using endogenously occurring pyruvate analogues and UV-induced nonpersistent radicals.NMR in biomedicine · 2021Article
- Acquisition strategies for spatially resolved magnetic resonance detection of hyperpolarized nuclei.Magma (New York, N.Y.) · 2020Review
- Simultaneous TJournal of magnetic resonance (San Diego, Calif. : 1997) · 2020Article
- Magnetic resonance imaging with hyperpolarized agents: methods and applications.Physics in medicine and biology · 2017Review
- Metabolomic profiling of CSF in multiple sclerosis and neuromyelitis optica spectrum disorder by nuclear magnetic resonance.PloS one · 2017Article
- MR Molecular Imaging of Brain Cancer Metabolism Using Hyperpolarized 13C Magnetic Resonance Spectroscopy.Topics in magnetic resonance imaging : TMRI · 2016Review
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
purposeThe purpose of this study was to investigate (13) C hyperpolarization of α-ketobutyrate (αKB), an endogenous molecular analog of pyruvate, and its in vivo enzymatic conversion via lactate dehydrogenase (LDH) using localized MR spectroscopy.
methodsHyperpolarized (HP) (13) C MR experiments were conducted using [(13) C]αKB with rats in vivo and with isolated LDH enzyme in vitro, along with comparative experiments using [(13) C]pyruvate. Based on differences in the kinetics of its reaction with individual LDH isoforms, HP [(13) C]αKB was investigated as a novel MR probe, with added specificity for activity of LDHB-expressed H ("heart"-type) subunits of LDH (e.g., constituents of LDH-1 isoform).
resultsComparable T1 and polarization values to pyruvate were attained (T1 = 52 s at 3 tesla [T], polarization = 10%, at C1 ). MR experiments showed rapid enzymatic conversion with substantially increased specificity. Formation of product HP [(13) C]α-hydroxybutyrate (αHB) from αKB in vivo was increased 2.7-fold in cardiac slabs relative to liver and kidney slabs. In vitro studies resulted in 5.0-fold higher product production from αKB with bovine heart LDH-1, as compared with pyruvate.
conclusionsHP [(13) C]αKB may be a useful MR probe of cardiac metabolism and other applications where the role of H subunits of LDH is significant (e.g., renal cortex and brain).
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