ArticlePlant biotechnology journal2016
Metabolic engineering of sugarcane to accumulate energy-dense triacylglycerols in vegetative biomass.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 65 papers, 1 of them a synthesis that pooled it.
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Who cites it
65 citing papers in PubMed, 1 synthesis or guideline pooled it, 189 citations in OpenAlex.
- A toolkit for plant lipid engineering: Surveying the efficacies of lipogenic factors for accumulating specialty lipids.Frontiers in plant science · 2022Pooled it
- Decoding the Mystery of Sugarcane Genome-Based Breeding: Current Advancements, Strategies, and Future Challenges.Plant, cell & environment · 2026Review
- Multisite Field Evaluation of Oil Accumulation and Agronomic Performance in Grain and Sweet Sorghums Engineered for Lipid Hyperaccumulation.Plant biotechnology journal · 2026Article
- A comprehensive evaluation of metabolically engineered Cynara cardunculus calli as platform for valuable fatty acid derivatives production.Scientific reports · 2025Article
- Leaf oil under stress: divergent lipid synthesis and stabilization strategies in tobacco.Plant physiology · 2025Article
- Enhanced seed oil content by overexpressing LPAAT and WRI1 genes in tobacco plant.Plant cell reports · 2025Article
- The transcription factor CeWRI3 functions in TAG accumulation through activating CeOLE2 in Cyperus esculentus.Plant cell reports · 2025Article
- Water stress enhances triacylglycerol accumulation via different mechanisms in wild-type and transgenic high-leaf oil tobacco.Plant physiology · 2025Article
- RNAi and genome editing of sugarcane: Progress and prospects.The Plant journal : for cell and molecular biology · 2025Review
- Development of vegetative oil sorghum: From lab-to-field.Plant biotechnology journal · 2025Article
- Resourceful and economical designing of fermentation medium for lab and commercial strains of yeast from alternative feedstock: 'transgenic oilcane'.Biotechnology for biofuels and bioproducts · 2025Article
- Toward sustainable crops: integrating vegetative (non-seed) lipid storage, carbon-nitrogen dynamics, and redox regulation.Frontiers in plant science · 2025Review
- Exploiting lipid droplet metabolic pathway to foster lipid production: oleosin in focus.Plant cell reports · 2024Review
- CRISPR/Cas technology: fueling the future of Biofuel production with sugarcane.Functional & integrative genomics · 2024Review
- Genetic Engineering for Enhancing Sugarcane Tolerance to Biotic and Abiotic Stresses.Plants (Basel, Switzerland) · 2024Review
- Intron-mediated enhancement of DIACYLGLYCEROL ACYLTRANSFERASE1 expression in energycane promotes a step change for lipid accumulation in vegetative tissues.Biotechnology for biofuels and bioproducts · 2023Article
- An end-to-end pipeline for succinic acid production at an industrially relevant scale using Issatchenkia orientalis.Nature communications · 2023Article
- Microbiome differences in sugarcane and metabolically engineered oilcane accessions and their implications for bioenergy production.Biotechnology for biofuels and bioproducts · 2023Article
- Engineering triacylglycerol accumulation in duckweed (Lemna japonica).Plant biotechnology journal · 2023Article
- Long read transcriptome sequencing of a sugarcane hybrid and its progenitors,Frontiers in plant science · 2023Article
5 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
12 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Elevating the lipid content in vegetative tissues has emerged as a new strategy for increasing energy density and biofuel yield of crops. Storage lipids in contrast to structural and signaling lipids are mainly composed of glycerol esters of fatty acids, also known as triacylglycerol (TAG). TAGs are one of the most energy-rich and abundant forms of reduced carbon available in nature. Therefore, altering the carbon-partitioning balance in favour of TAG in vegetative tissues of sugarcane, one of the highest yielding biomass crops, is expected to drastically increase energy yields. Here we report metabolic engineering to elevate TAG accumulation in vegetative tissues of sugarcane. Constitutive co-expression of WRINKLED1 (WRI1), diacylglycerol acyltransferase1-2 (DGAT1-2) and oleosin1 (OLE1) and simultaneous cosuppression of ADP-glucose pyrophosphorylase (AGPase) and a subunit of the peroxisomal ABC transporter1 (PXA1) in transgenic sugarcane elevated TAG accumulation in leaves or stems by 95- or 43-fold to 1.9% or 0.9% of dry weight (DW), respectively, while expression or suppression of one to three of the target genes increased TAG levels by 1.5- to 9.5-fold. Accumulation of TAG in vegetative progeny plants was consistent with the results from primary transgenics and contributed to a total fatty acid content of up to 4.7% or 1.7% of DW in mature leaves or stems, respectively. Lipid droplets were visible within mesophyll cells of transgenic leaves by confocal fluorescence microscopy. These results provide the basis for optimizations of TAG accumulation in sugarcane and other high yielding biomass grasses and will open new prospects for biofuel applications.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.