ArticleDiabetologia2015
Metabolite profiling in plasma and tissues of ob/ob and db/db mice identifies novel markers of obesity and type 2 diabetes.
Article in Diabetologia, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed, 159 citations in OpenAlex.
- Article
- Experimental models in diabetes research.Laboratory animal research · 2026Review
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- The impact of type 2 diabetes on tendon material properties and locomotor function in the db/db mouse.Experimental physiology · 2026Article
- Relevance and Safe Utilization of Amino Acids in Supplements for Human Nutrition: Lessons from Clinical and Preclinical Studies.Nutrients · 2026Review
- Enhancing metabolite coverage using dedicated mobile phases for individual polarity modes in HILIC-MS.Analytical and bioanalytical chemistry · 2026Article
- Obesity and Cognitive Function: Leptin Role Through Blood-Brain Barrier and Hippocampus.Molecular neurobiology · 2025Review
- Comparative Analysis of Animal Models in Wound Healing Research and the Utility for Humanized Mice Models.Advances in wound care · 2025Review
- Differential susceptibility of immunodeficient mice to MPXV infection and the impact of various inoculation routes.Virologica Sinica · 2025Article
- Defective protein persulfidation is involved in obesity associated skeletal muscle dysfunction: role of SIRT-1.Redox biology · 2025Article
- Branched-chain amino acid metabolism: Pathophysiological mechanism and therapeutic intervention in metabolic diseases.Obesity reviews : an official journal of the International Association for the Study of Obesity · 2025Review
- NFAT5 exacerbates β-cell ferroptosis by suppressing the transcription of PRDX2 in obese type 2 diabetes mellitus.Cellular and molecular life sciences : CMLS · 2025Article
- A Comprehensive Metabolomic and Microbial Analysis Following Dietary Amino Acid Reduction in Mice.Metabolites · 2024Article
- Features of Metabolism and Its Regulation in the Dynamics of Experimental Models of Metabolic Disorders.Bulletin of experimental biology and medicine · 2024Article
- Glycerol Kinase Drives Hepatic de novo Lipogenesis and Triglyceride Synthesis in Nonalcoholic Fatty Liver by Activating SREBP-1c Transcription, Upregulating DGAT1/2 Expression, and Promoting Glycerol Metabolism.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2024Article
- Leptin signalling regulates transcriptional differences in granulosa cells from genetically obese mice but not the activation of NLRP3 inflammasome.Scientific reports · 2024Article
- Article
- Elevated BCAA catabolism reverses the effect of branched-chain ketoacids on glucose transport in mTORC1-dependent manner in L6 myotubes.Journal of nutritional science · 2024Article
- Impact of a High-Fat Diet at a Young Age on Wound Healing in Mice.International journal of molecular sciences · 2023Article
- Review
22 more citing papers are in PubMed but not listed here.
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aims/hypothesisMetabolomics approaches in humans have identified around 40 plasma metabolites associated with insulin resistance (IR) and type 2 diabetes, which often coincide with those for obesity. We aimed to separate diabetes-associated from obesity-associated metabolite alterations in plasma and study the impact of metabolically important tissues on plasma metabolite concentrations.
methodsTwo obese mouse models were studied; one exclusively with obesity (ob/ob) and another with type 2 diabetes (db/db). Both models have impaired leptin signalling as a cause for obesity, but the different genetic backgrounds determine the susceptibility to diabetes. In these mice, we profiled plasma, liver, skeletal muscle and adipose tissue via semi-quantitative GC-MS and quantitative liquid chromatography (LC)-MS/MS for a wide range of metabolites.
resultsMetabolite profiling identified 24 metabolites specifically associated with diabetes but not with obesity. Among these are known markers such as 1,5-anhydro-D-sorbitol, 3-hydroxybutyrate and the recently reported marker glyoxylate. New metabolites in the diabetic model were lysine, O-phosphotyrosine and branched-chain fatty acids. We also identified 33 metabolites that were similarly altered in both models, represented by branched-chain amino acids (BCAA) as well as glycine, serine, trans-4-hydroxyproline, and various lipid species and derivatives. Correlation analyses showed stronger associations for plasma amino acids with adipose tissue metabolites in db/db mice compared with ob/ob mice, suggesting a prominent contribution of adipose tissue to changes in plasma in a diabetic state. CONCLUSIONS/
interpretationBy studying mice with metabolite signatures that resemble obesity and diabetes in humans, we have found new metabolite entities for validation in appropriate human cohorts and revealed their possible tissue of origin.
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