Evidence map›Paper›PMID 26057830›Full record

ArticleeLife2015

Circular RNA biogenesis can proceed through an exon-containing lariat precursor.

Steven P Barrett, Peter L Wang, Julia Salzman

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in eLife, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 277 papers, 2 of them syntheses that pooled it.

0numbers the graph read from it
0cells of the map it votes in
277citing papers in PubMed, 2 pooled it
15.8field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

277 citing papers in PubMed, 2 syntheses or guidelines pooled it, 433 citations in OpenAlex.

  1. Pooled it
  2. The Emerging Role of Circular RNAs in Prostate Cancer: A Systematic Review.Frontiers in cell and developmental biology · 2021
    Pooled it
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217 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 1 institution in 1 country.

Steven P BarrettDepartment of Biochemistry, Stanford University School of Medicine, Stanford, United States.
Peter L WangDepartment of Biochemistry, Stanford University School of Medicine, Stanford, United States.
Julia SalzmanDepartment of Biochemistry, Stanford University School of Medicine, Stanford, United States.
Stanford University · US

Funding

Discovering genomic rearrangements under selection in serious ovarian cancerR00CA168987 · NCI · STANFORD UNIVERSITY · PI SALZMAN, JULIA · 2014 to 2016
$704k
NCI NIH HHS R00 CA168987NCI NIH HHS R00, CA168987-03
6 · The paper itself

Abstract

Pervasive expression of circular RNA is a recently discovered feature of eukaryotic gene expression programs, yet its function remains largely unknown. The presumed biogenesis of these RNAs involves a non-canonical 'backsplicing' event. Recent studies in mammalian cell culture posit that backsplicing is facilitated by inverted repeats flanking the circularized exon(s). Although such sequence elements are common in mammals, they are rare in lower eukaryotes, making current models insufficient to describe circularization. Through systematic splice site mutagenesis and the identification of splicing intermediates, we show that circular RNA in Schizosaccharomyces pombe is generated through an exon-containing lariat precursor. Furthermore, we have performed high-throughput and comprehensive mutagenesis of a circle-forming exon, which enabled us to discover a systematic effect of exon length on RNA circularization. Our results uncover a mechanism for circular RNA biogenesis that may account for circularization in genes that lack noticeable flanking intronic secondary structure.

Indexed as

Nucleic Acid ConformationRNA SplicingDNA Mutational AnalysisRNASchizosaccharomycesRNAchromosomescircular RNAdebranchinggeneslariatsRNA-seqsplicingS. pombe

Identifiers

PMID26057830
PMCPMC4479058
OpenAlexW2100155969

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.