ArticleActa crystallographica. Section F, Structural biology communications2015
Crystallization and preliminary crystallographic analysis of latent, active and recombinantly expressed aurone synthase, a polyphenol oxidase, from Coreopsis grandiflora.
Article in Acta crystallographica. Section F, Structural biology communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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21 citing papers in PubMed, 31 citations in OpenAlex.
- Historical Insights Into the Purification of Polyphenol Oxidase From Plants.Food science & nutrition · 2025Review
- Speciation atlas of polyoxometalates in aqueous solutions.Science advances · 2023Article
- Biochemical characterization of Dimocarpus longan polyphenol oxidase provides insights into its catalytic efficiency.Scientific reports · 2022Article
- Speciation of Transition-Metal-Substituted Keggin-Type Silicotungstates Affected by the Co-crystallization Conditions with Proteinase K.Inorganic chemistry · 2021Article
- A Peptide-Induced Self-Cleavage Reaction Initiates the Activation of Tyrosinase.Angewandte Chemie (International ed. in English) · 2019Article
- Biochemical and structural characterization of tomato polyphenol oxidases provide novel insights into their substrate specificity.Scientific reports · 2019Article
- The P-type ATPase inhibiting potential of polyoxotungstates.Metallomics : integrated biometal science · 2018Article
- The antibacterial activity of polyoxometalates: structures, antibiotic effects and future perspectives.Chemical communications (Cambridge, England) · 2018Article
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- Three recombinantly expressed apple tyrosinases suggest the amino acids responsible for mono- versus diphenolase activity in plant polyphenol oxidases.Scientific reports · 2017Article
- In crystallo activity tests with latent apple tyrosinase and two mutants reveal the importance of the mutated sites for polyphenol oxidase activity.Acta crystallographica. Section F, Structural biology communications · 2017Article
- Ten Good Reasons for the Use of the Tellurium-Centered Anderson-Evans Polyoxotungstate in Protein Crystallography.Accounts of chemical research · 2017Review
- Oxidative Cyclization in Natural Product Biosynthesis.Chemical reviews · 2017Review
- 2-Hydroxypyridine-ACS medicinal chemistry letters · 2017Article
- In situ formation of the first proteinogenically functionalized [TeWChemical communications (Cambridge, England) · 2016Article
- [Ni(OH)3W6O18(OCH2)3CCH2OH](4-): the first tris-functionalized Anderson-type heteropolytungstate.Chemical communications (Cambridge, England) · 2016Article
- Aurone synthase is a catechol oxidase with hydroxylase activity and provides insights into the mechanism of plant polyphenol oxidases.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Large-Scale Recombinant Expression and Purification of Human Tyrosinase Suitable for Structural Studies.PloS one · 2016Article
- The Synthesis and Characterization of Aromatic Hybrid Anderson-Evans POMs and their Serum Albumin Interactions: The Shift from Polar to Hydrophobic Interactions.Chemistry (Weinheim an der Bergstrasse, Germany) · 2015Article
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3 authors at 1 institution in 1 country.
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Abstract
Aurone synthase (AUS), a member of a novel group of plant polyphenol oxidases (PPOs), catalyzes the oxidative conversion of chalcones to aurones. Two active cgAUS1 (41.6 kDa) forms that differed in the level of phosphorylation or sulfation as well as the latent precursor form (58.9 kDa) were purified from the petals of Coreopsis grandiflora. The differing active cgAUS1 forms and the latent cgAUS1 as well as recombinantly expressed latent cgAUS1 were crystallized, resulting in six different crystal forms. The active forms crystallized in space groups P2(1)2(1)2(1) and P12(1)1 and diffracted to ∼ 1.65 Å resolution. Co-crystallization of active cgAUS1 with 1,4-resorcinol led to crystals belonging to space group P3(1)21. The crystals of latent cgAUS1 belonged to space group P12(1)1 and diffracted to 2.50 Å resolution. Co-crystallization of recombinantly expressed pro-AUS with the hexatungstotellurate(VI) salt Na6[TeW6O24] within the liquid-liquid phase separation zone significantly improved the quality of the crystals compared with crystals obtained without hexatungstotellurate(VI).
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