ArticleInternational journal of molecular sciences2015
De Novo Characterization of Flower Bud Transcriptomes and the Development of EST-SSR Markers for the Endangered Tree Tapiscia sinensis.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Article
- Transcriptomic and Metabolomic Analysis Unravels the Molecular Regulatory Mechanism of Fatty Acid Biosynthesis inInternational journal of molecular sciences · 2022Article
- The de novo genome assembly of Tapiscia sinensis and the transcriptomic and developmental bases of androdioecy.Horticulture research · 2020Article
- The complete chloroplast genome sequence ofMitochondrial DNA. Part B, Resources · 2020Article
- Genome survey sequencing and identification of genomic SSR markers for Rhododendron micranthum.Bioscience reports · 2020Article
- De novo assembly of transcriptomes, mining, and development of novel EST-SSR markers in Curcuma alismatifolia (Zingiberaceae family) through Illumina sequencing.Scientific reports · 2019Article
- Development and Characterization of High-Throughput EST-Based SSR Markers forMolecules (Basel, Switzerland) · 2018Article
- De Novo Transcriptome Assembly and Population Genetic Analyses for an Endangered Chinese EndemicGenes · 2018Article
- Article
- Carbohydrate metabolism and gene regulation during anther development in an androdioecious tree, Tapiscia sinensis.Annals of botany · 2017Article
- De novo assembly and characterization of the floral transcriptome of an economically important tree species, Lindera glauca (Lauraceae), including the development of EST-SSR markers for population genetics.Molecular biology reports · 2016Article
- De novo Transcriptome Assembly of Floral Buds of Pineapple and Identification of Differentially Expressed Genes in Response to Ethephon Induction.Frontiers in plant science · 2016Article
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6 authors.
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Abstract
Tapiscia sinensis Oliv (Tapisciaceae) is an endangered species native to China famous for its androdioecious breeding system. However, there is a lack of genomic and transcriptome data on this species. In this study, the Tapiscia sinensis transcriptomes from two types of sex flower buds were sequenced. A total of 97,431,176 clean reads were assembled into 52,169 unigenes with an average length of 1116 bp. Through similarity comparison with known protein databases, 36,662 unigenes (70.27%) were annotated. A total of 10,002 (19.17%) unigenes were assigned to 124 pathways using the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway database. Additionally, 10,371 simple sequence repeats (SSRs) were identified in 8608 unigenes, with 16,317 pairs of primers designed for applications. 150 pairs of primers were chosen for further validation, and the 68 pairs (45.5%) were able to produce clear polymorphic bands. Six polymorphic SSR markers were used to Bayesian clustering analysis of 51 T. sinensis individuals. This is the first report to provide transcriptome information and to develop large-scale SSR molecular markers for T. sinensis. This study provides a valuable resource for conservation genetics and functional genomics research on T. sinensis for future work.
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