ArticleActa crystallographica. Section D, Biological crystallography2015
Structure determination of an integral membrane protein at room temperature from crystals in situ.
Article in Acta crystallographica. Section D, Biological crystallography, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed.
- High-Resolution Cryo-Electron Microscopy Structure Determination ofInternational journal of molecular sciences · 2024Article
- Discovery of a Potent, Selective, and Cell-Active SPIN1 Inhibitor.Journal of medicinal chemistry · 2024Article
- xia2.multiplex: a multi-crystal data-analysis pipeline.Acta crystallographica. Section D, Structural biology · 2022Article
- FMX - the Frontier Microfocusing Macromolecular Crystallography Beamline at the National Synchrotron Light Source II.Journal of synchrotron radiation · 2021Article
- Changes in Membrane Protein Structural Biology.Biology · 2020Review
- Low-doseIUCrJ · 2020Article
- High-throughput in situ experimental phasing.Acta crystallographica. Section D, Structural biology · 2020Article
- Scaling diffraction data in the DIALS software package: algorithms and new approaches for multi-crystal scaling.Acta crystallographica. Section D, Structural biology · 2020Article
- Article
- Article
- Determination of Patterson group symmetry from sparse multi-crystal data sets in the presence of an indexing ambiguity.Acta crystallographica. Section D, Structural biology · 2018Article
- On the state of crystallography at the dawn of the electron microscopy revolution.Current opinion in structural biology · 2017Review
- Review
- A Novel Approach to Data Collection for Difficult Structures: Data Management for Large Numbers of Crystals with the BLEND Software.Crystals · 2017Article
- New approaches towards the understanding of integral membrane proteins: A structural perspective on G protein-coupled receptors.Protein science : a publication of the Protein Society · 2017Review
- Signatures of Mechanosensitive Gating.Biophysical journal · 2017Article
- Room temperature structure of human IgG4-Fc from crystals analysed in situ.Molecular immunology · 2017Article
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Current advances in synchrotron radiation instrumentation for MX experiments.Archives of biochemistry and biophysics · 2016Review
- Applications of thin-film sandwich crystallization platforms.Acta crystallographica. Section F, Structural biology communications · 2016Article
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Abstract
The structure determination of an integral membrane protein using synchrotron X-ray diffraction data collected at room temperature directly in vapour-diffusion crystallization plates (in situ) is demonstrated. Exposing the crystals in situ eliminates manual sample handling and, since it is performed at room temperature, removes the complication of cryoprotection and potential structural anomalies induced by sample cryocooling. Essential to the method is the ability to limit radiation damage by recording a small amount of data per sample from many samples and subsequently assembling the resulting data sets using specialized software. The validity of this procedure is established by the structure determination of Haemophilus influenza TehA at 2.3 Å resolution. The method presented offers an effective protocol for the fast and efficient determination of membrane-protein structures at room temperature using third-generation synchrotron beamlines.
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