ArticlePloS one2015
RNAi-Mediated Knock-Down of transformer and transformer 2 to Generate Male-Only Progeny in the Oriental Fruit Fly, Bactrocera dorsalis (Hendel).
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
33 citing papers in PubMed, 69 citations in OpenAlex.
- Molecular characterization of transformer, transformer-2, and doublesex genes in the carambola fruit fly, Bactrocera carambolae, revealing potential for genetic pest management.Insect science · 2026Article
- Synthetic biology approaches to generate temperature-sensitive alleles for the Sterile Insect Technique.Insect science · 2026Review
- The transformer gene controls sexual development in Drosophila suzukii.Insect science · 2026Article
- Article
- A Comparative Assessment of Self-limiting Genetic Control Strategies for Population Suppression.Molecular biology and evolution · 2025Article
- Gene drive and genetic sex conversion in the global agricultural pest Ceratitis capitata.Nature communications · 2024Article
- Article
- The Roles ofInsects · 2023Article
- Review
- Identification of sex-specific markers based on gene expression in adult Colorado potato beetles.Journal of insect science (Online) · 2023Article
- Review
- The Sex Determination Cascade in the Silkworm.Genes · 2021Review
- Female-to-male sex conversion in Ceratitis capitata by CRISPR/Cas9 HDR-induced point mutations in the sex determination gene transformer-2.Scientific reports · 2020Article
- The transformer-2 and fruitless characterisation with developmental expression profiles of sex-determining genes in Bactrocera dorsalis and B. correcta.Scientific reports · 2020Article
- Transcriptome Analysis of the Oriental Fruit FlyInsects · 2020Article
- Male-biased Adult Production of the Striped Fruit Fly,Insects · 2020Article
- Identification of sex determination genes and their evolution in Phlebotominae sand flies (Diptera, Nematocera).BMC genomics · 2019Article
- RNAi-Mediated Knockdown ofInsects · 2019Article
- Two of the three Transformer-2 genes are required for ovarian development in Aedes albopictus.Insect biochemistry and molecular biology · 2019Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The transformer (tra) gene appears to act as the genetic switch that promotes female development by interaction with the transformer2 (tra-2) gene in several dipteran species including the Medfly, housefly and Drosophila melanogaster. In this study, we describe the isolation, expression and function of tra and tra-2 in the economically important agricultural pest, the oriental fruit fly, Bactrocera dorsalis (Hendel). Bdtra and Bdtra-2 are similar to their homologs from other tephritid species. Bdtra demonstrated sex-specific transcripts: one transcript in females and two transcripts in males. In contrast, Bdtra-2 only had one transcript that was common to males and females, which was transcribed continuously in different adult tissues and developmental stages. Bdtra-2 and the female form of Bdtra were maternally inherited in eggs, whereas the male form of Bdtra was not detectable until embryos of 1 and 2 h after egg laying. Function analyses of Bdtra and Bdtra-2 indicated that both were indispensable for female development, as nearly 100% males were obtained with embryonic RNAi against either Bdtra or Bdtra-2. The fertility of these RNAi-generated males was subsequently tested. More than 80% of RNAi-generated males could mate and the mated females could lay eggs, but only 40-48.6% males gave rise to progeny. In XX-reversed males and intersex individuals, no clear female gonadal morphology was observed after dissection. These results shed light on the development of a genetic sexing system with male-only release for this agricultural pest.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.