ArticleJournal of experimental neuroscience2015
A New Technique for Collection of Cerebrospinal Fluid in Rat Pups.
Article in Journal of experimental neuroscience, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Hypoxic-Ischemic Insult Alters Polyamine and Neurotransmitter Abundance in the Specific Neonatal Rat Brain Subregions.ACS chemical neuroscience · 2024Article
- GABA Administration Ameliorates the Toxicity of Doxorubicin on CSF and the Brain of Albino Rats.Annals of neurosciences · 2024Article
- Population Pharmacokinetic Modeling of Free Plasma and Free Brain Concentrations of Ceftaroline in Healthy and Methicillin-Resistant Staphylococcus aureus-Infected Wistar Rats.Antimicrobial agents and chemotherapy · 2023Article
- Altered offspring neurodevelopment in an L-NAME-induced preeclampsia rat model.Frontiers in pediatrics · 2023Article
- Effects of Hypothermia and Allopurinol on Oxidative Status in a Rat Model of Hypoxic Ischemic Encephalopathy.Antioxidants (Basel, Switzerland) · 2021Article
- GT1b functions as a novel endogenous agonist of toll-like receptor 2 inducing neuropathic pain.The EMBO journal · 2020Article
- Potential biomarkers for neuroinflammation and neurodegeneration at short and long term after neonatal hypoxic-ischemic insult in rat.Journal of neuroinflammation · 2019Article
- Magnesium Sulfate Provides Neuroprotection in Eclampsia-Like Seizure Model by Ameliorating Neuroinflammation and Brain Edema.Molecular neurobiology · 2017Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundNeuroprotective strategies to prevent or decrease brain injury in hypoxic ischemic newborns are one of the main research lines in neonatology. Animal models have been used to assess the efficiency of new therapeutic strategies. Brain damage biomarkers in cerebrospinal fluid (CSF) are frequently used to evaluate the outcome at the bedside. Despite the importance of this approach in clinical practice, there are many difficulties in using it in small animals. The aim of this paper was to describe a new technique for collecting CSF in rat pups. Furthermore the reference values of S100β protein levels, commonly used in common clinical practice, were analyzed in animals between 7 to 12 days.
methods42 Wistar rat pups aged 7 to 12 days were used. CSF was obtained by direct puncture of the cisterna magna with a 24-gauge needle. S100β protein levels were determined with enzyme-linked immunosorbent assay (ELISA).
resultsCSF was successfully obtained in 96% of the cases, with an average amount of 21.28 μl (5-40 μl). Normal values for S100β were described. HI animals presented higher S100β values than controls.
conclusionsA simple, reproducible technique for CSF collection in rat pups has been described. This new method will allow study of brain injury biomarkers in newborn hypoxic ischemic animal models.
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