ArticleInfection and immunity2015
A Genome-Wide Screen Reveals that the Vibrio cholerae Phosphoenolpyruvate Phosphotransferase System Modulates Virulence Gene Expression.
Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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20 citing papers in PubMed, 34 citations in OpenAlex.
- Pathogenic Characteristics of Shrimp Early Mortality Syndrome (EMS)-Causing Vibrio parahaemolyticus: A Comparative Transcriptomic Study Suggests the Relationship Between Metabolic Fitness and Virulence Gene Expression.Environmental microbiology reports · 2025Article
- Role of the TPR family protein VPA1365 in regulating type III secretion system 2 and virulence inApplied and environmental microbiology · 2025Article
- Review
- Transcriptome Analysis Reveals the Effect of Low NaCl Concentration on Osmotic Stress and Type III Secretion System inInternational journal of molecular sciences · 2023Article
- Association of gut microbiome and metabolites with onset and treatment response of patients with pemphigus vulgaris.Frontiers in immunology · 2023Article
- The Role of Nutrients and Nutritional Signals in the Pathogenesis of Vibrio cholerae.Advances in experimental medicine and biology · 2023Article
- A Novel Transcription Factor VPA0041 Was Identified to Regulate the Swarming Motility inPathogens (Basel, Switzerland) · 2022Article
- A dual-function RNA balances carbon uptake and central metabolism in Vibrio cholerae.The EMBO journal · 2021Article
- Carbohydrate Metabolism Affects Macrophage-Mediated Killing of Enterococcus faecalis.mSystems · 2021Article
- Transporters of glucose and other carbohydrates in bacteria.Pflugers Archiv : European journal of physiology · 2020Review
- Parallel quorum-sensing system in Vibrio cholerae prevents signal interference inside the host.PLoS pathogens · 2020Article
- A Vibrio cholerae BolA-Like Protein Is Required for Proper Cell Shape and Cell Envelope Integrity.mBio · 2019Article
- Transposon mutagenesis and identification of mutated genes in growth-delayed Edwardsiella ictaluri.BMC microbiology · 2019Article
- Critical role for a promoter discriminator in RpoS control of virulence in Edwardsiella piscicida.PLoS pathogens · 2018Article
- Identification of the Regulon of AphB and Its Essential Roles in LuxR and Exotoxin Asp Expression in the Pathogen Vibrio alginolyticus.Journal of bacteriology · 2017Article
- Plasma and Mucosal Immunoglobulin M, Immunoglobulin A, and Immunoglobulin G Responses to the Vibrio cholerae O1 Protein Immunome in Adults With Cholera in Bangladesh.The Journal of infectious diseases · 2017Article
- Systematic genetic dissection of PTS in Vibrio cholerae uncovers a novel glucose transporter and a limited role for PTS during infection of a mammalian host.Molecular microbiology · 2017Article
- Article
- A Cytosine Methyltransferase Modulates the Cell Envelope Stress Response in the Cholera Pathogen [corrected].PLoS genetics · 2015Article
- MlrA, a MerR family regulator inGut microbesArticle
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Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
Abstract
Diverse environmental stimuli and a complex network of regulatory factors are known to modulate expression of Vibrio cholerae's principal virulence factors. However, there is relatively little known about how metabolic factors impinge upon the pathogen's well-characterized cascade of transcription factors that induce expression of cholera toxin and the toxin-coregulated pilus (TCP). Here, we used a transposon insertion site (TIS) sequencing-based strategy to identify new factors required for expression of tcpA, which encodes the major subunit of TCP, the organism's chief intestinal colonization factor. Besides identifying most of the genes known to modulate tcpA expression, the screen yielded ptsI and ptsH, which encode the enzyme I (EI) and Hpr components of the V. cholerae phosphoenolpyruvate phosphotransferase system (PTS). In addition to reduced expression of TcpA, strains lacking EI, Hpr, or the associated EIIA(Glc) protein produced less cholera toxin (CT) and had a diminished capacity to colonize the infant mouse intestine. The PTS modulates virulence gene expression by regulating expression of tcpPH and aphAB, which themselves control expression of toxT, the central activator of virulence gene expression. One mechanism by which PTS promotes virulence gene expression appears to be by modulating the amounts of intracellular cyclic AMP (cAMP). Our findings reveal that the V. cholerae PTS is an additional modulator of the ToxT regulon and demonstrate the potency of loss-of-function TIS sequencing screens for defining regulatory networks.
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