ArticleProceedings of the National Academy of Sciences of the United States of America2015
Bioimage analysis of Shigella infection reveals targeting of colonic crypts.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
36 citing papers in PubMed.
- Shigella sonnei: epidemiology, evolution, pathogenesis, resistance and host interactions.Nature reviews. Microbiology · 2025Review
- The promiscuous biotin ligase TurboID reveals the proxisome of the T3SS chaperone IpgC inmSphere · 2024Article
- A foundational approach to culture and analyze malnourished organoids.Gut microbes · 2023Article
- Ascorbate deficiency increases progression of shigellosis in guinea pigs and mice infection models.Gut microbes · 2023Article
- Production of highly immunogenic and safe Triton X-100 produced bacterial ghost vaccine against Shigella flexneri 2b serotype.Gut pathogens · 2023Article
- 4D live imaging and computational modeling of a functional gut-on-a-chip evaluate how peristalsis facilitates enteric pathogen invasion.Science advances · 2022Article
- Gastric stem cells promote inflammation and gland remodeling in response to Helicobacter pylori via Rspo3-Lgr4 axis.The EMBO journal · 2022Article
- The VirF21:VirF30 protein ratio is affected by temperature and impacts Shigella flexneri host cell invasion.FEMS microbiology letters · 2022Article
- Tissue compartmentalization enablesProceedings of the National Academy of Sciences of the United States of America · 2021Article
- Biofilm Formation and Virulence of Shigella flexneri Are Modulated by pH of Gastrointestinal Tract.Infection and immunity · 2021Article
- Mechanisms controlling bacterial infection in myeloid cells under hypoxic conditions.Cellular and molecular life sciences : CMLS · 2021Review
- An Oral Inoculation Infant Rabbit Model formBio · 2020Article
- Immunoglobulins, Mucosal Immunity and Vaccination in Teleost Fish.Frontiers in immunology · 2020Review
- The virulence domain of Shigella IcsA contains a subregion with specific host cell adhesion function.PloS one · 2020Article
- Shigella-mediated oxygen depletion is essential for intestinal mucosa colonization.Nature microbiology · 2019Article
- Mapping of Shigella flexneri's tissue distribution and type III secretion apparatus activity during infection of the large intestine of guinea pigs.Pathogens and disease · 2019Article
- Article
- Review
- Review
- Control of bacterial colonization in the glands and crypts.Current opinion in microbiology · 2019Review
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Few studies within the pathogenic field have used advanced imaging and analytical tools to quantitatively measure pathogenicity in vivo. In this work, we present a novel approach for the investigation of host-pathogen processes based on medium-throughput 3D fluorescence imaging. The guinea pig model for Shigella flexneri invasion of the colonic mucosa was used to monitor the infectious process over time with GFP-expressing S. flexneri. A precise quantitative imaging protocol was devised to follow individual S. flexneri in a large tissue volume. An extensive dataset of confocal images was obtained and processed to extract specific quantitative information regarding the progression of S. flexneri infection in an unbiased and exhaustive manner. Specific parameters included the analysis of S. flexneri positions relative to the epithelial surface, S. flexneri density within the tissue, and volume of tissue destruction. In particular, at early time points, there was a clear association of S. flexneri with crypts, key morphological features of the colonic mucosa. Numerical simulations based on random bacterial entry confirmed the bias of experimentally measured S. flexneri for early crypt targeting. The application of a correlative light and electron microscopy technique adapted for thick tissue samples further confirmed the location of S. flexneri within colonocytes at the mouth of crypts. This quantitative imaging approach is a novel means to examine host-pathogen systems in a tailored and robust manner, inclusive of the infectious agent.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.