ArticleScientific reports2015
Osteocyte specific responses to soluble and mechanical stimuli in a stem cell derived culture model.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 44 citations in OpenAlex.
- Sclerostin and parathyroid hormone responses to acute whole-body vibration and resistance exercise in young women.Journal of bone and mineral metabolism · 2019Trial
- Bone Multicellular Unit on a Chip (BMU-Chip) Subjected to Cyclic Mechanical Loading.ACS biomaterials science & engineering · 2026Article
- Loss of the auxiliary αJBMR plus · 2024Article
- Bone-homing metastatic breast cancer cells impair osteocytes' mechanoresponse in a 3D loading model.Heliyon · 2023Article
- Bone Formation in 2D Culture of Primary Cells.JBMR plus · 2023Article
- The skeleton in a physical world.Experimental biology and medicine (Maywood, N.J.) · 2022Review
- Osteocytes and Estrogen Deficiency.Current osteoporosis reports · 2021Review
- Generation of two multipotent mesenchymal progenitor cell lines capable of osteogenic, mature osteocyte, adipogenic, and chondrogenic differentiation.Scientific reports · 2021Article
- The HIF-PHI BAY 85-3934 (Molidustat) Improves Anemia and Is Associated With Reduced Levels of Circulating FGF23 in a CKD Mouse Model.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2021Article
- Article
- Cell Sources for Human In vitro Bone Models.Current osteoporosis reports · 2021Review
- Exercise and Diet: Uncovering Prospective Mediators of Skeletal Fragility in Bone and Marrow Adipose Tissue.Current osteoporosis reports · 2020Review
- Mechanical suppression of breast cancer cell invasion and paracrine signaling to osteoclasts requires nucleo-cytoskeletal connectivity.Bone research · 2020Article
- A Novel 3D Osteoblast and Osteocyte Model Revealing Changes in Mineralization and Pro-osteoclastogenic Paracrine Signaling During Estrogen Deficiency.Frontiers in bioengineering and biotechnology · 2020Article
- Influence of Low-Magnitude High-Frequency Vibration on Bone Cells and Bone Regeneration.Frontiers in bioengineering and biotechnology · 2020Review
- Combating osteoporosis and obesity with exercise: leveraging cell mechanosensitivity.Nature reviews. Endocrinology · 2019Review
- Recovery of stem cell proliferation by low intensity vibration under simulated microgravity requires LINC complex.NPJ microgravity · 2019Article
- Bench-to-bedside strategies for osteoporotic fracture: From osteoimmunology to mechanosensation.Bone research · 2019Review
- Bio-Inspired Micropatterned Platforms Recapitulate 3D Physiological Morphologies of Bone and Dentinal Cells.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Engineering in-vitro stem cell-based vascularized bone models for drug screening and predictive toxicology.Stem cell research & therapy · 2018Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
Abstract
Studying osteocyte behavior in culture has proven difficult because these embedded cells require spatially coordinated interactions with the matrix and surrounding cells to achieve the osteocyte phenotype. Using an easily attainable source of bone marrow mesenchymal stem cells, we generated cells with the osteocyte phenotype within two weeks. These "stem cell derived-osteocytes" (SCD-O) displayed stellate morphology and lacunocanalicular ultrastructure. Osteocytic genes Sost, Dmp1, E11, and Fgf23 were maximally expressed at 15 days and responded to PTH and 1,25(OH)2D3. Production of sclerostin mRNA and protein, within 15 days of culture makes the SCD-O model ideal for elucidating regulatory mechanisms. We found sclerostin to be regulated by mechanical factors, where low intensity vibration significantly reduced Sost expression. Additionally, this model recapitulates sclerostin production in response to osteoactive hormones, as PTH or LIV repressed secretion of sclerostin, significantly impacting Wnt-mediated Axin2 expression, via β-catenin signaling. In summary, SCD-O cells produce abundant matrix, rapidly attain the osteocyte phenotype, and secrete functional factors including sclerostin under non-immortalized conditions. This culture model enables ex vivo observations of osteocyte behavior while preserving an organ-like environment. Furthermore, as marrow-derived mesenchymal stem cells can be obtained from transgenic animals; our model enables study of genetic control of osteocyte behaviors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.