ArticleThe Journal of biological chemistry2015
Molecular Anatomy of ParA-ParA and ParA-ParB Interactions during Plasmid Partitioning.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 38 citations in OpenAlex.
- Article
- Molecular basis of ParA ATPase activation by the CTPase ParB during bacterial chromosome segregation.Nature communications · 2025Article
- Mutational analysis of the F plasmid partitioning protein ParA reveals residues required for oligomerization and plasmid maintenance.Nucleic acids research · 2025Article
- Epidemiological and biological characteristics of IncR plasmids as multihost antibiotic resistance carriers.Frontiers in microbiology · 2025Review
- Three factors ParA, TipN, and DnaA-mediated chromosome replication initiation are contributors of centromere segregation inMolecular biology of the cell · 2024Article
- TipN's involvement with centromere segregation in Caulobacter crescentus.bioRxiv : the preprint server for biology · 2023Article
- Kinetic principles of ParA2-ATP cycling guide dynamic subcellular localizations in Vibrio cholerae.Nucleic acids research · 2023Article
- Diverse Partners of the Partitioning ParB Protein in Pseudomonas aeruginosa.Microbiology spectrum · 2023Article
- Distinct architectural requirements for theeLife · 2022Article
- Persistence and Dissemination Capacities of aAntibiotics (Basel, Switzerland) · 2022Article
- Catching a Walker in the Act-DNA Partitioning by ParA Family of Proteins.Frontiers in microbiology · 2022Review
- Dissection of the ATPase active site of McdA reveals the sequential steps essential for carboxysome distribution.Molecular biology of the cell · 2021Article
- The structure of the bacterial DNA segregation ATPase filament reveals the conformational plasticity of ParA upon DNA binding.Nature communications · 2021Article
- The emergence of phase separation as an organizing principle in bacteria.Biophysical journal · 2021Review
- Mechanisms for Chromosome Segregation in Bacteria.Frontiers in microbiology · 2021Review
- Nonspecific DNA binding by P1 ParA determines the distribution of plasmid partition and repressor activities.The Journal of biological chemistry · 2020Article
- Bacterial chromosome segregation by the ParABS system.Open biology · 2020Review
- Molecular architecture of the DNA-binding sites of the P-loop ATPases MipZ and ParA from Caulobacter crescentus.Nucleic acids research · 2020Article
- Crystal structures of HpSoj-DNA complexes and the nucleoid-adaptor complex formation in chromosome segregation.Nucleic acids research · 2019Article
- Genomic and functional characterisation of IncX3 plasmids encoding blaScientific reports · 2018Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Firmicutes multidrug resistance inc18 plasmids encode parS sites and two small homodimeric ParA-like (δ2) and ParB-like (ω2) proteins to ensure faithful segregation. Protein ω2 binds to parS DNA, forming a short left-handed helix wrapped around the full parS, and interacts with δ2. Protein δ2 interacts with ω2 and, in the ATP-bound form, binds to nonspecific DNA (nsDNA), forming small clusters. Here, we have mapped the ω2·δ2 and δ2·δ2 interacting domains in the δ2 that are adjacent to but distinct from each other. The δ2 nsDNA binding domain is essential for stimulation of ω2·parS-mediated ATP hydrolysis. From the data presented here, we propose that δ2 interacts with ATP, nsDNA, and with ω2 bound to parS at near equimolar concentrations, facilitating a δ2 structural transition. This δ2 "activated" state overcomes its impediment in ATP hydrolysis, with the subsequent release of both of the proteins from nsDNA (plasmid unpairing).
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