ArticleParasites & vectors2015
Molecular insights into a tetraspanin in the hydatid tapeworm Echinococcus granulosus.
Article in Parasites & vectors, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 27 citations in OpenAlex.
- Molecular characterization and B-cell epitope analysis of the TSP11 gene inParasitology · 2024Article
- Silencing TUBB3 Expression Destroys the Tegument and Flame Cells ofAnimals : an open access journal from MDPI · 2022Article
- Molecular Characterization of a Tetraspanin TSP11 Gene inFrontiers in veterinary science · 2021Article
- Tropical Medicine in China: Bibliometric Analysis Based on Web of Science (2010-2019).Journal of tropical medicine · 2021Article
- Transcriptomic Analysis of the Early Strobilar Development ofPathogens (Basel, Switzerland) · 2020Article
- Biological and morphological consequences of dsRNA-induced suppression of tetraspanin mRNA in developmental stages of Echinococcus granulosus.Parasites & vectors · 2020Article
- Identification of combined T-cell and B-cell reactiveAnnals of translational medicine · 2019Article
- Cloning, expression, characterization, and immunological properties of citrate synthase from Echinococcus granulosus.Parasitology research · 2019Article
- Calmodulin-specific small interfering RNA induces consistent expression suppression and morphological changes in Echinococcus granulosus.Scientific reports · 2019Article
- Extracellular vesicles from Echinococcus granulosus larval stage: Isolation, characterization and uptake by dendritic cells.PLoS neglected tropical diseases · 2019Article
- Molecular and biochemical characterization of calmodulin from Echinococcus granulosus.Parasites & vectors · 2017Article
- Conservation and diversification of small RNA pathways within flatworms.BMC evolutionary biology · 2017Article
- Molecular Characteristics and Serodiagnostic Potential of Dihydrofolate Reductase from Echinococcus granulosus.Scientific reports · 2017Article
- Molecular characterization and serodiagnostic potential of a novel dithiol glutaredoxin 1 from Echinococcus granulosus.Parasites & vectors · 2016Article
- Analysis on Gene Expression Profile in Oncospheres and Early Stage Metacestodes from Echinococcus multilocularis.PLoS neglected tropical diseases · 2016Article
- Characterization of a Secretory Annexin in Echinococcus granulosus.The American journal of tropical medicine and hygiene · 2016Article
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Authors and funding
10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundCystic echinococcosis (hydatid disease), caused by the tapeworm Echinococcus granulosus (class Cestoda; family Taeniidae), is a neglected tropical disease that results in morbidity and mortality in millions of humans, as well as in huge economic losses in the livestock industry globally. Proteins from the tetraspanin family in parasites have recently become regarded as crucial molecules in interaction with hosts in parasitism and are therefore suitable for the development of vaccines and diagnostic agents. However, no information is available to date on E. granulosus tetraspanin.
methodsIn this study, a uroplakin-I-like tetraspanin (Eg-TSP1) of E. granulosus was cloned and expressed in E. coli. The immunolocalization of Eg-TSP1 in different life stages of E. granulosus was determined using specific polyclonal antibody. The antibody and cytokine profiles of mice that immunized with recombinant Eg-TSP1 (rEg-TSP1) were measured for the immunogenicity analysis of this protein. Additionally, we use RNA interference method to explore the biological function of Eg-TSP1 in larva of E. granulosus.
resultsImmunofluorescence analysis showed that endogenous Eg-TSP1 mainly localized in the tegument of larvae and adults. Significantly elevated levels of antibodies IgG1 and IgG2a and of cytokines IFN-γ and IL-12 were observed in the sera of mice after immunization with rEg-TSP1, suggesting a typical T helper (Th)1-mediated immune response elicited by rEg-TSP1. On further probing the role of Eg-TSP1 in E. granulosus by RNA interference, we found that a thinner tegmental distal cytoplasm was induced in protoscoleces treated with siRNA-132 compared to controls.
conclusionsThis is the first report characterizing a tetraspanin from the tapeworm E. granulosus. Our results suggest that Eg-TSP1 is associated with biogenesis of the tegument and maintenance of structural integrity of E. granulosus and could therefore be a candidate intervention target for control of hydatid disease.
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