ArticleVirulence2015
In vitro Paracoccidioides brasiliensis biofilm and gene expression of adhesins and hydrolytic enzymes.
Article in Virulence, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 42 citations in OpenAlex.
- Antimicrobial Peptides: A Promising Alternative to Conventional Antimicrobials for Combating Polymicrobial Biofilms.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Diagnosis of Human Endemic Mycoses Caused by Thermally Dimorphic Fungi: From Classical to Molecular Methods.Journal of fungi (Basel, Switzerland) · 2024Review
- Common virulence factors betweenCurrent research in microbial sciences · 2024Review
- Article
- Antifungal Activity of Chitosan againstJournal of fungi (Basel, Switzerland) · 2023Article
- Alginate-Based 3D A549 Cell Culture Model to StudyJournal of fungi (Basel, Switzerland) · 2023Article
- Connection between MHC class II binding and aggregation propensity: The antigenic peptide 10 ofComputational and structural biotechnology journal · 2023Article
- Biofilm Formation by Chromoblastomycosis FungiJournal of fungi (Basel, Switzerland) · 2022Article
- Peptides derived from gp43, the most antigenic protein from Paracoccidioides brasiliensis, form amyloid fibrils in vitro: implications for vaccine development.Scientific reports · 2021Article
- Article
- Silver(I) and Copper(II) Complexes of 1,10-Phenanthroline-5,6-Dione AgainstFrontiers in microbiology · 2021Article
- Gene expression of Paracoccidioides virulence factors after interaction with macrophages and fibroblasts.Memorias do Instituto Oswaldo Cruz · 2021Article
- Heat Shock Protein 60, Insights to Its Importance inFrontiers in cellular and infection microbiology · 2020Article
- Biofilm Formation byFrontiers in microbiology · 2020Article
- Dynamics of Mono- and Dual-Species Biofilm Formation and Interactions BetweenFrontiers in microbiology · 2020Article
- Article
- Secreted aspartyl proteinase (PbSap) contributes to the virulence of Paracoccidioides brasiliensis infection.PLoS neglected tropical diseases · 2018Article
- In Vivo Paracoccidioides sp. Biofilm on Vascular Prosthesis.Mycopathologia · 2017Article
- Fungal Biofilms and Polymicrobial Diseases.Journal of fungi (Basel, Switzerland) · 2017Review
- Antifungal Therapy: New Advances in the Understanding and Treatment of Mycosis.Frontiers in microbiology · 2017Review
Corrections and comments
- Erratum issuedErratum.2015
Authors and funding
7 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Paracoccidioides species are dimorphic fungi that initially infect the lungs but can also spread throughout the body. The spreading infection is most likely due to the formation of a biofilm that makes it difficult for the host to eliminate the infection. Biofilm formation is crucial for the development of infections and confines the pathogen to an extracellular matrix. Its presence is associated with antimicrobial resistance and avoidance of host defenses. This current study provides the first description of biofilm formation by Paracoccidioides brasiliensis (Pb18) and an analysis of gene expression, using real-time PCR, associated with 3 adhesins and 2 hydrolytic enzymes that could be associated with the virulence profile. Biofilm formation was analyzed using fluorescence microscopy, scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM). Metabolic activity was determined using the XTT reduction assay. P. brasiliensis was able to form mature biofilm in 144 h with a thickness of 100 μm. The presence of a biofilm was found to be associated with an increase in the expression of adhesins and enzymes. GP43, enolase, GAPDH and aspartyl proteinase genes were over-expressed, whereas phospholipase was down-regulated in biofilm. The characterization of biofilm formed by P. brasiliensis may contribute to a better understanding of the pathogenesis of paracoccidioidomycosis as well as the search for new therapeutic alternatives; while improving the effectiveness of treatment.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.