ArticleAntimicrobial agents and chemotherapy2015
Characterization of BKC-1 class A carbapenemase from Klebsiella pneumoniae clinical isolates in Brazil.
Article in Antimicrobial agents and chemotherapy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 84 citations in OpenAlex.
- Detection of virulent Klebsiella pneumoniae strains causing intestinal and extraintestinal infections during the 80s and 90s in Brazil.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2024Article
- Emergence of multidrug-resistantOne health (Amsterdam, Netherlands) · 2024Article
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- Capture ELISA for KPC Detection in Gram-Negative Bacilli: Development and Standardisation.Microorganisms · 2023Article
- Latent antibiotic resistance genes are abundant, diverse, and mobile in human, animal, and environmental microbiomes.Microbiome · 2023Article
- Phenotypic and genotypic antimicrobial resistance correlation and plasmid characterization inFrontiers in public health · 2023Article
- Characterization of a Carbapenem-Resistant BKC-1-Producing Clinical Isolate Belonging to the Pseudomonas putida Group from Brazil.Antimicrobial agents and chemotherapy · 2022Article
- The biogenesis of β-lactamase enzymes.Microbiology (Reading, England) · 2022Review
- Co-production of Classes A and B Carbapenemases BKC-1 and VIM-2 in a Clinical Pseudomonas Putida Group Isolate from Brazil.Current microbiology · 2022Article
- Role of ISAntimicrobial agents and chemotherapy · 2022Article
- DeepBL: a deep learning-based approach for in silico discovery of beta-lactamases.Briefings in bioinformatics · 2021Article
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- Frequency and Diversity of HybridMicroorganisms · 2021Article
- BKC-2, a New BKC Variant Detected in MCR-9.1-Producing Enterobacter hormaechei subsp.Antimicrobial agents and chemotherapy · 2021Article
- Extensively drug-resistant IMP-16-producing Pseudomonas monteilii isolated from cerebrospinal fluid.Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases · 2021Article
- The Class A Carbapenemases BKC-1 and GPC-1 Both Originate from the Bacterial GenusAntimicrobial agents and chemotherapy · 2020Article
- Article
- Biochemical Activity of Vaborbactam.Antimicrobial agents and chemotherapy · 2020Article
- Identification of plasmid IncQ1 and NTEKPC-IId harboring bla KPC-2 in isolates from Klebsiella pneumoniae infections in patients from Recife-PE, Brazil.Revista da Sociedade Brasileira de Medicina Tropical · 2020Article
- Genetic Diversity, Biochemical Properties, and Detection Methods of Minor Carbapenemases in Enterobacterales.Frontiers in medicine · 2020Review
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Three Klebsiella pneumoniae clinical isolates demonstrating carbapenem resistance were recovered from different patients hospitalized at two medical centers in São Paulo, Brazil. Resistance to all β-lactams, quinolones, and some aminoglycosides was observed for these isolates that were susceptible to polymyxin B. Carbapenem hydrolysis, which was inhibited by clavulanic acid, was observed for all K. pneumoniae isolates that belonged to the same pulsed-field gel electrophoresis (PFGE) type and a novel sequence type (ST), ST1781 (clonal complex 442 [CC442]). A 10-kb nonconjugative incompatibility group Q (IncQ) plasmid, denominated p60136, was transferred to Escherichia coli strain TOP10 cells by electroporation. The full sequencing of p60136 showed that it was composed of a mobilization system, ISKpn23, the phosphotransferase aph3A-VI, and a 941-bp open reading frame (ORF) that codified a 313-amino acid protein. This ORF was named bla BKC-1. Brazilian Klebsiella carbapenemase-1 (BKC-1) showed a pI of 6.0 and possessed the highest identity (63%) with a β-lactamase of Sinorhizobium meliloti, an environmental bacterium. Hydrolysis studies demonstrated that purified BKC-1 not only hydrolyzed carbapenems but also penicillins, cephalosporins, and monobactams. However, the carbapenems were less efficiently hydrolyzed due to their very low kcat values (0.0016 to 0.031 s(-1)). In fact, oxacillin was the best substrate for BKC-1 (kcat /Km , 53,522.6 mM(-1) s(-1)). Here, we report a new class A carbapenemase, confirming the diversity and rapid evolution of β-lactamases in K. pneumoniae clinical isolates.
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