ArticleBMC complementary and alternative medicine2015
Antifungal activity of berberine hydrochloride and palmatine hydrochloride against Microsporum canis -induced dermatitis in rabbits and underlying mechanism.
Article in BMC complementary and alternative medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 49 citations in OpenAlex.
- Berberine as a Promising Antifungal Agent: Insights into Molecular Mechanisms and Therapeutic Potential.ACS omega · 2026Review
- An Updated and Comprehensive Review ofMolecules (Basel, Switzerland) · 2026Review
- Inhibitory effects of berberine on Fonsecaea monophora in vitro and in vivo.BMC complementary medicine and therapies · 2025Article
- Inhibitory effects of berberine on fungal growth, biofilm formation, virulence, and drug resistance as an antifungal drug and adjuvant with prospects for future applications.World journal of microbiology & biotechnology · 2024Review
- Microscopic distribution of alkaloids in freeze-fixed stems ofFrontiers in plant science · 2023Article
- Palmatine Inhibits the Pathogenicity ofFoods (Basel, Switzerland) · 2022Article
- Antifungal Potential of Some Herb Decoctions and Essential Oils onHealthcare (Basel, Switzerland) · 2022Article
- Quantitative Analysis of Berberidis Cortex via Quantitative Analysis of Multicomponents by Single Marker (QAMS) Combined with Fingerprint and Chemometrics Methods.Journal of analytical methods in chemistry · 2022Article
- In vitro Antimicrobial Activity and the Mechanism of Berberine Against Methicillin-ResistantInfection and drug resistance · 2022Article
- Successful Treatment of Bullous Pemphigoid Lesions by Berberine Stamp Therapy: A Case Report and Literature Review.Frontiers in medicine · 2022Article
- Transcriptome sequencing revealed the inhibitory mechanism of ketoconazole on clinicalJournal of veterinary science · 2021Article
- Berberine-Loaded Thiolated Pluronic F127 Polymeric Micelles for Improving Skin Permeation and Retention.International journal of nanomedicine · 2020Article
- Antimicrobial characteristics of Berberine against prosthetic joint infection-related Staphylococcus aureus of different multi-locus sequence types.BMC complementary and alternative medicine · 2019Article
- Phellodendri Cortex: A Phytochemical, Pharmacological, and Pharmacokinetic Review.Evidence-based complementary and alternative medicine : eCAM · 2019Review
- In Vitro Study of Antiamoebic Activity of Methanol Extracts ofThe Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2018Article
- Strong Synergism of Palmatine and Fluconazole/Itraconazole Against Planktonic and Biofilm Cells ofFrontiers in microbiology · 2018Article
- In Vitro Activity of Berberine Alone and in Combination with Antifungal Drugs Against Planktonic Forms and Biofilms of Trichosporon Asahii.Mycopathologia · 2017Article
- Immunoregulatory effects of the traditional Dai prescription Yajieshaba on food allergic mice.Experimental and therapeutic medicine · 2017Article
- Gastroprotective effect of palmatine against acetic acid-induced gastric ulcers in rats.Journal of natural medicines · 2017Article
- A Novel Method to Mask the Bitter Taste of Berberine Hydrochloride: Powder Surface Modification.Pharmacognosy magazineArticle
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundPhellodendron amurense, exhibits antifungal activity mainly by bioactive components including berberine hydrochloride and palmatine hydrochloride. This study was conducted to evaluate the antifungal effects of berberine hydrochloride, palmatine hydrochloride, and a mixture of both substances against Microsporum canis in vivo and in vitro.
methodsThe minimal inhibitory concentrations (MICs) of monomers and clotrimazole were determined using 1.5 % tryptic soy agar. The effects of these drugs on Microsporum canis growth was detected by determining dry weight. Transmission electron microscopy (TEM) was performed to observe the effect of chemicals on cell ultrastructure. Differential mRNA expressions of eight genes of M. canis treated with berberine or palmatine or their combination at different time points were determined by real-time PCR. NADH enzyme concentration was also detected. Clinical evaluation via in-vivo antifungal assay was also performed. Skin histology PAS staining was also carried out.
resultsResults showed that MICs of berberine, palmatine and clotrimazole were 1, 1, and 0.015 mg/mL, respectively. No significant difference was observed among the growth curves of the three groups before 18 h was reached. TEM showed that these drugs could destroy the cell membrane and organelles of M. canis at different time points. After 30 h of incubation, relative mRNA expressions of the genes in the combined group were significantly higher than those in the other groups including the clotrimazole group (P < 0.05); Palmatine initially induced the mRNA up-regulation of PGAL4, FSH1, PQ-LRP, NADH1 and NDR in M. canis; by contrast, berberine maintained a high expression level of these genes to shorten fungal life cycle and eradicate M. canis. Clinical results showed that combined treatment was more effective than single administration of each monomer or clotrimazole. Hence, berberine mixed with palmatine significantly elicited antifungal activities and could be used to treat M. canis in rabbits.
conclusionThese results provide a comprehensive view of the mechanism of berberine and palmatine in anti-M. canis activity.
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