ArticleGenome biology2015
Drosophila Imp iCLIP identifies an RNA assemblage coordinating F-actin formation.
Article in Genome biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 41 citations in OpenAlex.
- Article
- Axonal RNA localization is essential for long-term memory.Nature communications · 2025Article
- Imp/IGF2BP and Syp/SYNCRIP temporal RNA interactomes uncover combinatorial networks of regulators ofScience advances · 2025Article
- An image-based RNAi screen identifies the EGFR signaling pathway as a regulator of Imp RNP granules.Journal of cell science · 2024Article
- In vivo PAR-CLIP (viP-CLIP) of liver TIAL1 unveils targets regulating cholesterol synthesis and secretion.Nature communications · 2023Article
- Molecular insights into RNA recognition and gene regulation by the TRIM-NHL protein Mei-P26.Life science alliance · 2022Article
- Pseudo-RNA-Binding Domains Mediate RNA Structure Specificity in Upstream of N-Ras.Cell reports · 2020Article
- Igf2bp3 maintains maternal RNA stability and ensures early embryo development in zebrafish.Communications biology · 2020Article
- Article
- Genome wide analysis of 3' UTR sequence elements and proteins regulating mRNA stability during maternal-to-zygotic transition in zebrafish.Genome research · 2019Article
- IMP regulates Kuzbanian to control the timing of Notch signalling inDevelopment (Cambridge, England) · 2019Article
- Article
- Adaptation of iCLIP to plants determines the binding landscape of the clock-regulated RNA-binding protein AtGRP7.Genome biology · 2017Article
- Optimization of PAR-CLIP for transcriptome-wide identification of binding sites of RNA-binding proteins.Methods (San Diego, Calif.) · 2017Review
- DO-RIP-seq to quantify RNA binding sites transcriptome-wide.Methods (San Diego, Calif.) · 2017Article
- Highly accessible AU-rich regions in 3' untranslated regions are hotspots for binding of regulatory factors.PLoS computational biology · 2017Article
- Mechanism of β-actin mRNA Recognition by ZBP1.Cell reports · 2017Article
- Quantifying RNA binding sites transcriptome-wide using DO-RIP-seq.RNA (New York, N.Y.) · 2017Article
- Putative RNA-directed adaptive mutations in cancer evolution.Transcription · 2016Article
- RNA assemblages orchestrate complex cellular processes.BioEssays : news and reviews in molecular, cellular and developmental biology · 2016Review
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
backgroundPost-transcriptional RNA regulons ensure coordinated expression of monocistronic mRNAs encoding functionally related proteins. In this study, we employ a combination of RIP-seq and short- and long-wave individual-nucleotide resolution crosslinking and immunoprecipitation (iCLIP) technologies in Drosophila cells to identify transcripts associated with cytoplasmic ribonucleoproteins (RNPs) containing the RNA-binding protein Imp.
resultsWe find extensive binding of Imp to 3' UTRs of transcripts that are involved in F-actin formation. A common denominator of the RNA-protein interface is the presence of multiple motifs with a central UA-rich element flanked by CA-rich elements. Experiments in single cells and intact flies reveal compromised actin cytoskeletal dynamics associated with low Imp levels. The former shows reduced F-actin formation and the latter exhibits abnormal neuronal patterning. This demonstrates a physiological significance of the defined RNA regulon.
conclusionsOur data imply that Drosophila Imp RNPs may function as cytoplasmic mRNA assemblages that encode proteins which participate in actin cytoskeletal remodeling. Thus, they may facilitate coordinated protein expression in sub-cytoplasmic locations such as growth cones.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.