ArticleBMC biotechnology2015
Continuous enhancement of iturin A production by Bacillus subtilis with a stepwise two-stage glucose feeding strategy.
Article in BMC biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 35 citations in OpenAlex.
- Application of Microorganisms for the Valorization of Side-Products of Rapeseed De-Oiling.Biomolecules · 2025Review
- Process optimized for production of iturin A in biofilm reactor by Bacillus velezensis ND.Bioprocess and biosystems engineering · 2024Article
- Creating Single-Cell Protein-ProducingScientifica · 2024Article
- Enhanced biosurfactant production by Bacillus subtilis SPB1 using developed fed-batch fermentation: effects of glucose levels and feeding systems.Bioprocess and biosystems engineering · 2023Article
- Article
- Enhanced iturin a production in a two-compartment biofilm reactor byFrontiers in bioengineering and biotechnology · 2023Article
- Characterization ofMicroorganisms · 2022Article
- Enhanced production of iturin A by strengthening fatty acid synthesis modules inFrontiers in bioengineering and biotechnology · 2022Article
- The unconventional adverse effects of fungal pretreatment on iturin A fermentation by Bacillus amyloliquefaciens CX-20.Microbial biotechnology · 2021Article
- Optimization of iturin A production from3 Biotech · 2021Article
- Key elements and regulation strategies of NRPSs for biosynthesis of lipopeptides by Bacillus.Applied microbiology and biotechnology · 2020Review
- Simultaneous hydrolysis with lipase and fermentation of rapeseed cake for iturin A production by Bacillus amyloliquefaciens CX-20.BMC biotechnology · 2019Article
- Influence of Phenotypic Dissociation in Bacillus subtilis Strain ET-1 on Iturin A Production.Current microbiology · 2019Article
- Establishment of a rapeseed meal fermentation model for iturin A production by Bacillus amyloliquefaciens CX-20.Microbial biotechnology · 2019Article
- Enhanced production of antifungal lipopeptide iturin A by Bacillus amyloliquefaciens LL3 through metabolic engineering and culture conditions optimization.Microbial cell factories · 2019Article
- Integrated strategy of temperature shift and mannitol feeding for enhanced production of echinocandin B by3 Biotech · 2019Article
- In Silico Discovery of Novel Ligands for Antimicrobial Lipopeptides for Computer-Aided Drug Design.Probiotics and antimicrobial proteins · 2018Review
Corrections and comments
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe lipopeptide antibiotic iturin A is an attractive biopesticide with the potential to replace chemical-based pesticides for controlling plant pathogens. However, its industrial fermentation has not been realized due to the high production costs and low product concentrations. This study aims to enhance iturin A production by performing a novel fermentation process with effective glucose feeding control using rapeseed meal as a low-cost nitrogen source.
resultsWe demonstrated that continuous and significant enhancement of iturin A production could be achieved by a novel two-stage glucose-feeding strategy with a stepwise decrease in feeding rate. Using this strategy, the ratio of spores to total cells could be maintained at a desirable/stable level of 0.80-0.86, and the reducing sugar concentration could be controlled at a low level of 2-3 g/L so that optimal substrate balance could be maintained throughout the feeding phase. As a result, the maximum iturin A concentration reached 1.12 g/L, which was two-fold higher than that of batch culture.
conclusionsThis is the first report which uses control of the glucose supply to improve iturin A production by fed-batch fermentation and identifies some important factors necessary to realize industrial iturin A production. This approach may also enhance the production of other useful secondary metabolites by Bacillus subtilis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.