ArticleNature structural & molecular biology2015
ATP binding drives substrate capture in an ECF transporter by a release-and-catch mechanism.
Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Single-molecule visualization of ATP-induced dynamics of the subunit composition of an ECF transporter complex under turnover conditions.Nature communications · 2025Article
- Flavin transferase ApbE: From discovery to applications.The Journal of biological chemistry · 2025Review
- The S-component fold: a link between bacterial transporters and receptors.Communications biology · 2024Article
- Expulsion mechanism of the substrate-translocating subunit in ECF transporters.Nature communications · 2023Article
- Elongated Riboflavin-Producing Shewanella oneidensis in a Hybrid Biofilm Boosts Extracellular Electron Transfer.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2023Article
- Article
- Structural basis for inhibition of the drug efflux pump NorA from Staphylococcus aureus.Nature chemical biology · 2022Article
- RibU is an essential determinant ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Article
- Characterization of the Streptococcus mutansJournal of bacteriology · 2020Article
- Membrane mediated toppling mechanism of the folate energy coupling factor transporter.Nature communications · 2020Article
- Be Cautious with Crystal Structures of Membrane Proteins or Complexes Prepared in Detergents.Crystals · 2020Article
- Energy Cost of Force Production After a Stretch-Shortening Cycle in Skinned Muscle Fibers: Does Muscle Efficiency Increase?Frontiers in physiology · 2020Article
- Functional Prediction and Assignment ofFrontiers in genetics · 2020Article
- Metabolism of the Gram-Positive Bacterial PathogenMicrobiology spectrum · 2019Review
- The ATPase mechanism of UvrA2 reveals the distinct roles of proximal and distal ATPase sites in nucleotide excision repair.Nucleic acids research · 2019Article
- Cu Transport by the Extended Family of CcoA-like Transporters (CalT) in Proteobacteria.Scientific reports · 2019Article
- Functional and structural characterization of an ECF-type ABC transporter for vitamin B12.eLife · 2018Article
- Listeria monocytogenes cytosolic metabolism promotes replication, survival, and evasion of innate immunity.Cellular microbiology · 2017Review
- Uptake and Metabolism of Antibiotics Roseoflavin and 8-Demethyl-8-Aminoriboflavin in Riboflavin-Auxotrophic Listeria monocytogenes.Journal of bacteriology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
ECF transporters are a family of active transporters for vitamins. They are composed of four subunits: a membrane-embedded substrate-binding subunit (EcfS), a transmembrane coupling subunit (EcfT) and two ATP-binding-cassette ATPases (EcfA and EcfA'). We have investigated the mechanism of the ECF transporter for riboflavin from the pathogen Listeria monocytogenes, LmECF-RibU. Using structural and biochemical approaches, we found that ATP binding to the EcfAA' ATPases drives a conformational change that dissociates the S subunit from the EcfAA'T ECF module. Upon release from the ECF module, the RibU S subunit then binds the riboflavin transport substrate. We also find that S subunits for distinct substrates compete for the ATP-bound state of the ECF module. Our results explain how ECF transporters capture the transport substrate and reproduce the in vivo observations on S-subunit competition for which the family was named.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.