Evidence map›Paper›PMID 26052892›Full record

ArticleNature structural & molecular biology2015

The pilus usher controls protein interactions via domain masking and is functional as an oligomer.

Glenn T Werneburg, Nadine S Henderson, Erica B Portnoy, Samema Sarowar, Scott J Hultgren, Huilin Li, David G Thanassi

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Nature structural & molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed.

  1. Article
  2. Review
  3. Molecular Analysis ofArchives of Razi Institute · 2021
    Article
  4. Article
  5. Review
  6. Review
  7. Review
  8. Article
  9. Article
  10. Review
  11. Review
  12. UroPathogenicFrontiers in microbiology · 2017
    Review
  13. Review
  14. Article
  15. Article
  16. Article
  17. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Glenn T Werneburg1] Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA. [2] Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
Nadine S Henderson1] Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA. [2] Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
Erica B Portnoy1] Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA. [2] Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.
Samema Sarowar1] Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA. [2] Biosciences Department, Brookhaven National Laboratory, Upton, New York, USA.
Scott J Hultgren1] Department of Molecular Microbiology, Washington University School of Medicine, Saint Louis, Missouri, USA. [2] Center for Women's Infectious Disease Research, Washington University School of Medicine, Saint Louis, Missouri, USA.
Huilin Li1] Department of Biochemistry and Cell Biology, Stony Brook University, Stony Brook, New York, USA. [2] Biosciences Department, Brookhaven National Laboratory, Upton, New York, USA.
David G Thanassi1] Center for Infectious Diseases, Stony Brook University, Stony Brook, New York, USA. [2] Department of Molecular Genetics and Microbiology, Stony Brook University, Stony Brook, New York, USA.

Funding

MEDICAL SCIENTIST TRAINING PROGRAMT32GM008444 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI FROHMAN, MICHAEL A. · 1992 to 2024
$12.6M
Mechanism of the Usher in Assembly and Secretion of PiliR01GM062987 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI David G Thanassi · 2001 to 2026
$7.7M
CHAPERONE-ASSISTED PILI ASSEMBLY IN PATHOGENIC E. COLIR01AI029549 · NIAID · WASHINGTON UNIVERSITY · PI HULTGREN, SCOTT J., YUAN, PENG · 1991 to 2023
$5.7M
MOLECULAR AND CELLULAR BIOLOGY TRAINING PROGRAMT32GM008468 · NIGMS · STATE UNIVERSITY NEW YORK STONY BROOK · PI NEIMAN, AARON M · 1997 to 2016
$1.9M
Acquisition of an Applied Biosystems 4000 QTRAP Mass SpectrometerS10RR023680 · NCRR · STATE UNIVERSITY NEW YORK STONY BROOK · PI IDEN, CHARLES R · 2007 to 2007
$435k
Catalytic mechanism of the usher in pilus biogenesis by uropathogenic E. coliF30AI112252 · NIAID · STATE UNIVERSITY NEW YORK STONY BROOK · PI WERNEBURG, GLENN THOMAS · 2015 to 2018
$162k
NCRR NIH HHS S10 RR023680NCRR NIH HHS S10RR023680NIAID NIH HHS F30 AI112252NIAID NIH HHS F30AI112252NIAID NIH HHS R01 AI029549NIAID NIH HHS R01AI029549NIGMS NIH HHS R01 GM062987NIGMS NIH HHS R01GM062987NIGMS NIH HHS T32 GM008444NIGMS NIH HHS T32GM008444NIGMS NIH HHS T32 GM008468
6 · The paper itself

Abstract

The chaperone-usher (CU) pathway assembles organelles termed pili or fimbriae in Gram-negative bacteria. Type 1 pili expressed by uropathogenic Escherichia coli are prototypical structures assembled by the CU pathway. Biogenesis of pili by the CU pathway requires a periplasmic chaperone and an outer-membrane protein termed the usher (FimD). We show that the FimD C-terminal domains provide the high-affinity substrate-binding site but that these domains are masked in the resting usher. Domain masking requires the FimD plug domain, which serves as a switch controlling usher activation. We demonstrate that usher molecules can act in trans for pilus biogenesis, providing conclusive evidence for a functional usher oligomer. These results reveal mechanisms by which molecular machines such as the usher regulate and harness protein-protein interactions and suggest that ushers may interact in a cooperative manner during pilus assembly in bacteria.

Indexed as

Protein Interaction MapsBinding SitesEscherichia coliEscherichia coli ProteinsFimbriae ProteinsGene DeletionModels, MolecularProtein Structure, TertiaryEscherichia coli ProteinsFimbriae ProteinsfimD protein, E coli

Identifiers

PMID26052892
PMCPMC4496297

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.