ArticlePloS one2015
Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers, 1 of them a synthesis that pooled it.
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Who cites it
22 citing papers in PubMed, 1 synthesis or guideline pooled it, 34 citations in OpenAlex.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
- Early Regulatory and Th2-Associated Responses Shape Resistance toPathogens (Basel, Switzerland) · 2026Article
- Article
- Differential microRNA expression profiles and predicted miRNA-mRNA regulatory networks in human macrophage-like cells infected with Leishmania infantum.Scientific reports · 2026Article
- Mechanisms regulating host cell death duringmBio · 2024Review
- Expression of Network Medicine-Predicted Genes in Human Macrophages Infected withInternational journal of molecular sciences · 2024Article
- Transcriptomics analysis highlights potential ways in human pathogenesis in Leishmania braziliensis infected with the viral endosymbiont LRV1.PLoS neglected tropical diseases · 2024Article
- Transcriptional signatures in human macrophage-like cells infected by Leishmania infantum, Leishmania major and Leishmania tropica.PLoS neglected tropical diseases · 2024Article
- Transcriptomic Analysis in Human Macrophages Infected with Therapeutic Failure Clinical Isolates ofACS infectious diseases · 2022Article
- Expression Profile of Genes Related to the Th17 Pathway in Macrophages Infected byFrontiers in cellular and infection microbiology · 2022Article
- Encephalitozoon intestinalis Infection Impacts the Expression of Apoptosis-Related Genes in U937 Macrophage Cells.Acta parasitologica · 2021Article
- Gene expression patterns associated with Leishmania panamensis infection in macrophages from BALB/c and C57BL/6 mice.PLoS neglected tropical diseases · 2021Article
- Early Leukocyte Responses inFrontiers in cellular and infection microbiology · 2021Article
- Transcriptional analysis of THP-1 cells infected with Leishmania infantum indicates no activation of the inflammasome platform.PLoS neglected tropical diseases · 2020Article
- Leishmania donovani reduces the levels of retinoic acid-synthesizing enzymes in infected macrophages and favoring its own survival.Parasitology research · 2019Article
- Model-based Evaluation of Gene Expression Changes in Response toGene regulation and systems biology · 2019Review
- RNA-Seq Revealed Expression of Many Novel Genes Associated WithFrontiers in cellular and infection microbiology · 2019Article
- Leishmania donovani induced Unfolded Protein Response delays host cell apoptosis in PERK dependent manner.PLoS neglected tropical diseases · 2018Article
- Changes to cholesterol trafficking in macrophages by Leishmania parasites infection.MicrobiologyOpen · 2017Article
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Different Leishmania species cause distinct clinical manifestations of the infectious disease leishmaniasis. It is fundamentally important to understand the mechanisms governing the interaction between Leishmania and its host cell. Little is known about this interaction between Leishmania (Viannia) braziliensis and human macrophages. In this study, we aimed to identify differential gene expression between non-infected and L. (V) braziliensis-infected U937-derived macrophages. We deployed a whole human transcriptome microarray analysis using 72 hours post-infection samples and compared those samples with their non-infected counterparts. We found that 218 genes were differentially expressed between infected and non-infected macrophages. A total of 71.6% of these genes were down-regulated in the infected macrophages. Functional enrichment analyses identified the steroid and sterol/cholesterol biosynthetic processes between regulatory networks down-regulated in infected macrophages. RT-qPCR further confirmed this down-regulation in genes belonging to these pathways. These findings contrast with those from studies involving other Leishmania species at earlier infection stages, where gene up-regulation for this metabolic pathway has been reported. Sterol biosynthesis could be an important biological process associated with the expression profile of macrophages infected by L. (V.) braziliensis. Differential transcriptional results suggest a negative regulation of the genetic regulatory network involved in cholesterol biosynthesis.
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