Evidence map›Paper›PMID 26052433›Full record

ArticleRSC advances2014

Modification of PEGylated enzyme with glutaraldehyde can enhance stability while avoiding intermolecular crosslinking.

D W Ritter, J M Newton, M J McShane

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in RSC advances, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

D W Ritter *Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.
J M Newton *Department of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.
M J McShaneDepartment of Biomedical Engineering, Texas A&M University, College Station, TX 77843-3120, USA.

Funding

Implantable multi-analyte sensors for the continuous monitoring of body chemistriR01EB016414 · NIBIB · PROFUSA, INC. · PI MCSHANE, MIKE, WISNIEWSKI, NATALIE ANN · 2012 to 2015
$4.9M
NIBIB NIH HHS R01 EB016414
6 · The paper itself

Abstract

We demonstrate an enzyme stabilization approach whereby a model enzyme is PEGylated, followed by controlled chemical modification with glutaraldehyde. Using this stabilization strategy, size increases and aggregation due to intermolecular crosslinking are avoided. Immediately following synthesis, the PEGylated enzyme with and without glutaraldehyde modification possessed specific activities of 372.9 ± 20.68 U/mg and 373.9 ± 15.14 U/mg, respectively (

Identifiers

PMID26052433
PMCPMC4452374

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.