ArticleFrontiers in neuroanatomy2015
FIB/SEM technology and high-throughput 3D reconstruction of dendritic spines and synapses in GFP-labeled adult-generated neurons.
Article in Frontiers in neuroanatomy, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 48 papers.
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Who cites it
48 citing papers in PubMed, 73 citations in OpenAlex.
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- Reconstruction of ovine axonal cytoarchitecture enables more accurate models of brain biomechanics.Communications biology · 2022Article
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- Retinal horizontal cells use different synaptic sites for global feedforward and local feedback signaling.Current biology : CB · 2022Article
- Single-Neuron Labeling in Fixed Tissue and Targeted Volume Electron Microscopy.Frontiers in neuroanatomy · 2022Article
- Insight into the Characteristics of Novel Desmin-Immunopositive Perivascular Cells of the Anterior Pituitary Gland Using Transmission and Focused Ion Beam Scanning Electron Microscopy.International journal of molecular sciences · 2021Article
- Unique properties of dually innervated dendritic spines in pyramidal neurons of the somatosensory cortex uncovered by 3D correlative light and electron microscopy.PLoS biology · 2021Article
- Three-dimensional analysis of synaptic organization in the hippocampal CA1 field in Alzheimer's disease.Brain : a journal of neurology · 2021Article
- High levels of 27-hydroxycholesterol results in synaptic plasticity alterations in the hippocampus.Scientific reports · 2021Article
- Niwaki Instead of Random Forests: Targeted Serial Sectioning Scanning Electron Microscopy With Reimaging Capabilities for Exploring Central Nervous System Cell Biology and Pathology.Frontiers in neuroanatomy · 2021Review
- Three-Dimensional Structure of Dendritic Spines Revealed by Volume Electron Microscopy Techniques.Frontiers in neuroanatomy · 2021Article
- Application of the Mirror Technique for Three-Dimensional Electron Microscopy of Neurochemically Identified GABA-ergic Dendrites.Frontiers in neuroanatomy · 2021Article
- Glutamatergic Dysfunction and Synaptic Ultrastructural Alterations in Schizophrenia and Autism Spectrum Disorder: Evidence from Human and Rodent Studies.International journal of molecular sciences · 2020Review
- Cell Volume (3D) Correlative Microscopy Facilitated by Intracellular Fluorescent Nanodiamonds as Multi-Modal Probes.Nanomaterials (Basel, Switzerland) · 2020Article
- Two-Stage Alignment of FIB-SEM Images of Rock Samples.Journal of imaging · 2020Article
- Double staining method for array tomography using scanning electron microscopy.Applied microscopy · 2020Article
- Area-Specific Synapse Structure in Branched Posterior Nucleus Axons Reveals a New Level of Complexity in Thalamocortical Networks.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2020Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The fine analysis of synaptic contacts is usually performed using transmission electron microscopy (TEM) and its combination with neuronal labeling techniques. However, the complex 3D architecture of neuronal samples calls for their reconstruction from serial sections. Here we show that focused ion beam/scanning electron microscopy (FIB/SEM) allows efficient, complete, and automatic 3D reconstruction of identified dendrites, including their spines and synapses, from GFP/DAB-labeled neurons, with a resolution comparable to that of TEM. We applied this technology to analyze the synaptogenesis of labeled adult-generated granule cells (GCs) in mice. 3D reconstruction of dendritic spines in GCs aged 3-4 and 8-9 weeks revealed two different stages of dendritic spine development and unexpected features of synapse formation, including vacant and branched dendritic spines and presynaptic terminals establishing synapses with up to 10 dendritic spines. Given the reliability, efficiency, and high resolution of FIB/SEM technology and the wide use of DAB in conventional EM, we consider FIB/SEM fundamental for the detailed characterization of identified synaptic contacts in neurons in a high-throughput manner.
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