ArticleNature communications2015
Effective heritable gene knockdown in zebrafish using synthetic microRNAs.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
29 citing papers in PubMed, 49 citations in OpenAlex.
- Clinical relevance of zebrafish for gene variants testing. Proof-of-principle with SMN1/SMA.EMBO molecular medicine · 2026Article
- A recombinase-activated ribozyme to knock down endogenous gene expression in zebrafish.PLoS genetics · 2025Article
- Cre-Lox miRNA-delivery technology optimized for inducible microRNA and gene-silencing studies in zebrafish.Nucleic acids research · 2025Article
- Prosaposin maintains adult neural stem cells in a state associated with deep quiescence.Stem cell reports · 2024Article
- Modeling Spinal Muscular Atrophy in Zebrafish: Current Advances and Future Perspectives.International journal of molecular sciences · 2024Review
- NMJ Analyser: a novel method to quantify neuromuscular junction morphology in zebrafish.Bioinformatics (Oxford, England) · 2023Article
- Optimising the zebrafish Cre/Lox toolbox. Codon improved iCre, new gateway tools, Cre protein and guidelines.Frontiers in physiology · 2023Article
- The progress inSmall GTPases · 2022Review
- Modeling neuromuscular diseases in zebrafish.Frontiers in molecular neuroscience · 2022Review
- Cavin4 interacts with Bin1 to promote T-tubule formation and stability in developing skeletal muscle.The Journal of cell biology · 2021Article
- Reduced C9orf72 function leads to defective synaptic vesicle release and neuromuscular dysfunction in zebrafish.Communications biology · 2021Article
- Methods to Investigate miRNA Function: Focus on Platelet Reactivity.Thrombosis and haemostasis · 2021Review
- Neurexins in autism and schizophrenia-a review of patient mutations, mouse models and potential future directions.Molecular psychiatry · 2021Review
- Modular transient nanoclustering of activated β2-adrenergic receptors revealed by single-molecule tracking of conformation-specific nanobodies.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Inducible modulation of miR-204 levels in a zebrafish melanoma model.Biology open · 2020Article
- Potential of zebrafish as a model to characterise MicroRNA profiles in mechanically mediated joint degeneration.Histochemistry and cell biology · 2020Review
- ModellingBiomedicines · 2020Review
- The power and limitations of gene expression pathway analyses toward predicting population response to environmental stressors.Evolutionary applications · 2020Article
- Characterisation of d-Conotoxin TxVIA as a Mammalian T-Type Calcium Channel Modulator.Marine drugs · 2020Article
- The αScientific reports · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Although zebrafish is used to model human diseases through mutational and morpholino-based knockdown approaches, there are currently no robust transgenic knockdown tools. Here we investigate the knockdown efficiency of three synthetic miRNA-expressing backbones and show that these constructs can downregulate a sensor transgene with different degrees of potency. Using this approach, we reproduce spinal muscular atrophy (SMA) in zebrafish by targeting the smn1 gene. We also generate different transgenic lines, with severity and age of onset correlated to the level of smn1 inhibition, recapitulating for the first time the different forms of SMA in zebrafish. These lines are proof-of-concept that miRNA-based approaches can be used to generate potent heritable gene knockdown in zebrafish.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.