ArticleApplied microbiology and biotechnology2015
PEP3 overexpression shortens lag phase but does not alter growth rate in Saccharomyces cerevisiae exposed to acetic acid stress.
Article in Applied microbiology and biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 22 citations in OpenAlex.
- The endophyte Klebsiella pasteurii BDA134-6 isolated from African rice colonizes durum wheat plants helping them cope with water shortage stress.Scientific reports · 2025Article
- Acid-tolerant bacteria and prospects in industrial and environmental applications.Applied microbiology and biotechnology · 2023Review
- How adaptive laboratory evolution can boost yeast tolerance to lignocellulosic hydrolyses.Current genetics · 2022Review
- The toxicity of neodymium and genome-scale genetic screen of neodymium-sensitive gene deletion mutations in the yeast Saccharomyces cerevisiae.Environmental science and pollution research international · 2022Article
- Candida glabrata Yap6 Recruits Med2 To Alter Glycerophospholipid Composition and Develop Acid pH Stress Resistance.Applied and environmental microbiology · 2020Article
- Microbial lag phase can be indicative of, or independent from, cellular stress.Scientific reports · 2020Article
- Microbial response to acid stress: mechanisms and applications.Applied microbiology and biotechnology · 2020Review
- Adaptive Response and Tolerance to Acetic Acid inFrontiers in microbiology · 2018Review
- Omics analysis of acetic acid tolerance in Saccharomyces cerevisiae.World journal of microbiology & biotechnology · 2017Review
- A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations.Biotechnology for biofuels · 2016Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
In fungi, two recognized mechanisms contribute to pH homeostasis: the plasma membrane proton-pumping ATPase that exports excess protons and the vacuolar proton-pumping ATPase (V-ATPase) that mediates vacuolar proton uptake. Here, we report that overexpression of PEP3 which encodes a component of the HOPS and CORVET complexes involved in vacuolar biogenesis, shortened lag phase in Saccharomyces cerevisiae exposed to acetic acid stress. By confocal microscopy, PEP3-overexpressing cells stained with the vacuolar membrane-specific dye, FM4-64 had more fragmented vacuoles than the wild-type control. The stained overexpression mutant was also found to exhibit about 3.6-fold more FM4-64 fluorescence than the wild-type control as determined by flow cytometry. While the vacuolar pH of the wild-type strain grown in the presence of 80 mM acetic acid was significantly higher than in the absence of added acid, no significant difference was observed in vacuolar pH of the overexpression strain grown either in the presence or absence of 80 mM acetic acid. Based on an indirect growth assay, the PEP3-overexpression strain exhibited higher V-ATPase activity. We hypothesize that PEP3 overexpression provides protection from acid stress by increasing vacuolar surface area and V-ATPase activity and, hence, proton-sequestering capacity.
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