Evidence map›Paper›PMID 26050929›Full record

ArticleStem cell reports2015

The Genetic Landscape of Hematopoietic Stem Cell Frequency in Mice.

Xiaoying Zhou, Amanda L Crow, Jaana Hartiala, Tassja J Spindler, Anatole Ghazalpour, Lora W Barsky, Brian J Bennett, Brian W Parks, Eleazar Eskin, Rajan Jain and 4 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 31 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 1 country.

Xiaoying ZhouEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Amanda L CrowDepartment of Preventive Medicine and Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Jaana HartialaDepartment of Preventive Medicine and Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Tassja J SpindlerEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Anatole GhazalpourDepartments of Human Genetics, Medicine, and Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Lora W BarskyEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA.
Brian J BennettDepartment of Genetics and Nutrition Research Institute, University of North Carolina, Chapel Hill, Kannapolis, NC 28081, USA.
Brian W ParksDepartments of Human Genetics, Medicine, and Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Eleazar EskinDepartment of Computer Science and Inter-Departmental Program in Bioinformatics, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Rajan JainDepartment of Cell and Developmental Biology and Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Jonathan A EpsteinDepartment of Cell and Developmental Biology and Penn Cardiovascular Institute, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Aldons J LusisDepartments of Human Genetics, Medicine, and Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine at UCLA, Los Angeles, CA 90095, USA.
Gregor B AdamsEli and Edythe Broad Center for Regenerative Medicine and Stem Cell Research at USC, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. Electronic address: gregorad@med.usc.edu.
Hooman AllayeeDepartment of Preventive Medicine and Institute for Genetic Medicine, Keck School of Medicine, University of Southern California, Los Angeles, CA 90033, USA. Electronic address: hallayee@usc.edu.
University of California, Los Angeles · USUniversity of Southern California · USBroad Center · USUniversity of Pennsylvania · USDavid H. Murdock Research Institute · US

Funding

ULTRASTRUCTURE OF THE INTIMA IN EARLY LESION FORMATIONP01HL030568 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI FOGELMAN, ALAN M · 1985 to 2019
$55.2M
Systems genomics of metabolic syndrome traitsP01HL028481 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PAJUKANTA, PAIVI · 1985 to 2019
$50.2M
Translational Research Support CoreP30ES007048 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI Joseph Leo Wiemels · 1996 to 2026
$46.4M
Southern California Clinical and Translational Science InstituteUL1TR000130 · NCATS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI BUCHANAN, THOMAS A · 2012 to 2015
$36.0M
Training Grant in Genomic Analysis and InterpretationT32ES013678 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI GAUDERMAN, WILLIAM JAMES, MCCONNELL, ROB S · 2006 to 2023
$5.6M
An unusual homeobox gene required for heart developmentR01HL071546 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI EPSTEIN, JONATHAN A. · 2003 to 2017
$4.9M
INTEGRATIVE GENETIC APPROACHES TO GENE-AIR POLLUTION INTERACTIONS IN ASTHMAR01ES021801 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI AKBARI, OMID, ALLAYEE, HOOMAN · 2012 to 2016
$3.9M
Genetic and Environmental Determinants of Asthma EndophenotypesR01ES025786 · NIEHS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI AKBARI, OMID, ALLAYEE, HOOMAN · 2015 to 2019
$2.8M
Correcting for Population Structure in Gene-by-Environment Interaction StudiesR01ES022282 · NIEHS · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI ESKIN, ELEAZAR · 2013 to 2015
$1.2M
Investigating the role of Hopx in cardiac progenitor proliferationK08HL119553 · NHLBI · UNIVERSITY OF PENNSYLVANIA · PI JAIN, RAJAN · 2013 to 2017
$741k
Systems Genetics Analysis of Gene-by-Environment Interactions in ObesityK99HL123021 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI PARKS, BRIAN WESLEY · 2014 to 2015
$247k
High-Resolution Systems Genetic Studies of Atherosclerotic Lesion CompositionK99HL102223 · NHLBI · UNIVERSITY OF CALIFORNIA LOS ANGELES · PI BENNETT, BRIAN JOSEPH · 2011 to 2011
$108k
NCATS NIH HHS UL1 TR000130NCATS NIH HHS UL1TR000130NHLBI NIH HHS K08 HL119553NHLBI NIH HHS K99 HL102223NHLBI NIH HHS K99HL102223NHLBI NIH HHS K99 HL123021NHLBI NIH HHS K99HL123021NHLBI NIH HHS P01 HL028481NHLBI NIH HHS P01 HL030568NHLBI NIH HHS P01HL28481NHLBI NIH HHS P01HL30568NHLBI NIH HHS R01 HL071546NHLBI NIH HHS R01HL071546NIEHS NIH HHS 3R01ES021801-03S1NIEHS NIH HHS P30 ES007048NIEHS NIH HHS P30ES007048NIEHS NIH HHS R01 ES021801NIEHS NIH HHS R01ES021801NIEHS NIH HHS R01 ES022282NIEHS NIH HHS R01ES022282NIEHS NIH HHS R01 ES025786NIEHS NIH HHS T32 ES013678NIEHS NIH HHS T32ES013678
6 · The paper itself

Abstract

Prior efforts to identify regulators of hematopoietic stem cell physiology have relied mainly on candidate gene approaches with genetically modified mice. Here we used a genome-wide association study (GWAS) strategy with the hybrid mouse diversity panel to identify the genetic determinants of hematopoietic stem/progenitor cell (HSPC) frequency. Among 108 strains, we observed ∼120- to 300-fold variation in three HSPC populations. A GWAS analysis identified several loci that were significantly associated with HSPC frequency, including a locus on chromosome 5 harboring the homeodomain-only protein gene (Hopx). Hopx previously had been implicated in cardiac development but was not known to influence HSPC biology. Analysis of the HSPC pool in Hopx-/- mice demonstrated significantly reduced cell frequencies and impaired engraftment in competitive repopulation assays, thus providing functional validation of this positional candidate gene. These results demonstrate the power of GWAS in mice to identify genetic determinants of the hematopoietic system.

Indexed as

Genome-Wide Association StudyHematopoietic Stem CellsAnimalsCell LineageCell ProliferationHomeodomain ProteinsMiceMice, KnockoutHod protein, mouseHomeodomain Proteins

Identifiers

PMID26050929
PMCPMC4618249
OpenAlexW1923650300

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.