ArticleScientific reports2015
A Sweet Spot for Molecular Diagnostics: Coupling Isothermal Amplification and Strand Exchange Circuits to Glucometers.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed.
- Extraoral Detection of Biomarkers and Pathogens in Saliva: Comprehensive, Panoramic Review.Biosensors · 2026Review
- Article
- Cell-Free Gene Expression: Methods and Applications.Chemical reviews · 2025Review
- Advancements and applications of loop-mediated isothermal amplification technology: a comprehensive overview.Frontiers in microbiology · 2024Review
- Analysis of macerated ticks using Boolean logic gating colorimetric isothermal nucleic acid assays for Lyme Borrelia and Ixodes scapularis ticks.Scientific reports · 2023Article
- Plasmonic LAMP: Improving the Detection Specificity and Sensitivity for SARS-CoV-2 by Plasmonic Sensing of Isothermally Amplified Nucleic Acids.Small (Weinheim an der Bergstrasse, Germany) · 2022Article
- Electrochemical investigations for COVID-19 detection-A comparison with other viral detection methods.Chemical engineering journal (Lausanne, Switzerland : 1996) · 2021Article
- Article
- Hitting the diagnostic sweet spot: Point-of-care SARS-CoV-2 salivary antigen testing with an off-the-shelf glucometer.Biosensors & bioelectronics · 2021Article
- A glucose meter interface for point-of-care gene circuit-based diagnostics.Nature communications · 2021Article
- Developments in integrating nucleic acid isothermal amplification and detection systems for point-of-care diagnostics.Biosensors & bioelectronics · 2020Review
- One-tube smart genetic testing via coupling isothermal amplification and three-way nucleic acid circuit to glucometers.Analytica chimica acta · 2020Article
- Recent advances and perspectives of nucleic acid detection for coronavirus.Journal of pharmaceutical analysis · 2020Review
- Detection of Potato ring rot Pathogen Clavibacter michiganensis subsp. sepedonicus by Loop-mediated isothermal amplification (LAMP) assay.Scientific reports · 2019Article
- A signal-flexible gene diagnostic strategy coupling loop-mediated isothermal amplification with hybridization chain reaction.Analytica chimica acta · 2019Article
- Advances in Directly Amplifying Nucleic Acids from Complex Samples.Biosensors · 2019Review
- Find the right sample: A study on the versatility of saliva and urine samples for the diagnosis of emerging viruses.BMC infectious diseases · 2018Review
- Simultaneous Detection of Different Zika Virus Lineages via Molecular Computation in a Point-of-Care Assay.Viruses · 2018Article
- Direct nucleic acid analysis of mosquitoes for high fidelity species identification and detection of Wolbachia using a cellphone.PLoS neglected tropical diseases · 2018Article
- Biocomputing for Portable, Resettable, and Quantitative Point-of-Care Diagnostics: Making the Glucose Meter a Logic-Gate Responsive Device for Measuring Many Clinically Relevant Targets.Angewandte Chemie (International ed. in English) · 2018Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Strand exchange nucleic acid circuitry can be used to transduce isothermal nucleic acid amplification products into signals that can be readable on an off-the-shelf glucometer. Loop-mediated isothermal amplification (LAMP) is limited by the accumulation of non-specific products, but nucleic acid circuitry can be used to probe and distinguish specific amplicons. By combining this high temperature isothermal amplification method with a thermostable invertase, we can directly transduce Middle-East respiratory syndrome coronavirus and Zaire Ebolavirus templates into glucose signals, with a sensitivity as low as 20-100 copies/μl, equating to atto-molar (or low zepto-mole). Virus from cell lysates and synthetic templates could be readily amplified and detected even in sputum or saliva. An OR gate that coordinately triggered on viral amplicons further guaranteed fail-safe virus detection. The method describes has potential for accelerating point-of-care applications, in that biological samples could be applied to a transducer that would then directly interface with an off-the-shelf, approved medical device.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.