ArticleAnalytical biochemistry2015
An enhanced chimeric firefly luciferase-inspired enzyme for ATP detection and bioluminescence reporter and imaging applications.
Article in Analytical biochemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- A Computational Modeling of ADLumin Chemiluminescence: Oxygenation and Dioxetanone Formation.Journal of computational chemistry · 2026Article
- Bioluminescent enzymatic biosensors: ways to manage their characteristics.Biophysical reviews · 2025Review
- Fluorescence-Based Enzyme Activity Assay: Ascertaining the Activity and Inhibition of Endocannabinoid Hydrolytic Enzymes.International journal of molecular sciences · 2024Review
- Semirational engineering ofApplied and environmental microbiology · 2023Article
- Super-Stable Metal-Organic Framework (MOF)/Luciferase Paper-Sensing Platform for Rapid ATP Detection.Biosensors · 2023Article
- Novel Nanozeolitic Imidazolate Framework (ZIF-8)-Luciferase Biocomposite for Nanosensing Applications.Analytical chemistry · 2023Article
- Immobilization of Firefly Bioluminescent System: Development and Application of Reagents.Biosensors · 2022Article
- A Luciferase Mutant with Improved Brightness and Stability for Whole-Cell Bioluminescent Biosensors and In Vitro Biosensing.Biosensors · 2022Article
- Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer.Sensors (Basel, Switzerland) · 2022Article
- A higher spectral range of beetle bioluminescence with infraluciferin.Frontiers in bioengineering and biotechnology · 2022Article
- A New Ultrasensitive Bioluminescence-Based Method for Assaying Monoacylglycerol Lipase.International journal of molecular sciences · 2021Article
- Translating New Synthetic Biology Advances for Biosensing Into the Earth and Environmental Sciences.Frontiers in microbiology · 2020Review
- Development and Applications of Bioluminescent and Chemiluminescent Reporters and Biosensors.Annual review of analytical chemistry (Palo Alto, Calif.) · 2019Review
- A fluorometric displacement assay for adenosine triphosphate using layered cobalt(II) double hydroxide nanosheets.Mikrochimica acta · 2019Article
- Chemiluminescence and Bioluminescence Imaging for Biosensing and Therapy:Theranostics · 2019Review
- Signal amplification by strand displacement in a carbon dot based fluorometric assay for ATP.Mikrochimica acta · 2018Article
- A Firefly Luciferase Dual Color Bioluminescence Reporter Assay Using Two Substrates To Simultaneously Monitor Two Gene Expression Events.Scientific reports · 2018Article
- Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments.Analytical biochemistry · 2017Article
- A simple in vitro tumor chemosensitivity assay based on cell penetrating peptide tagged luciferase.PloS one · 2017Article
- Imaging Adenosine Triphosphate (ATP).The Biological bulletin · 2016Review
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Firefly luciferases, which emit visible light in a highly specific ATP-dependent process, have been adapted for a variety of applications, including gene reporter assays, whole-cell biosensor measurements, and in vivo imaging. We previously reported the approximately 2-fold enhanced activity and 1.4-fold greater bioluminescence quantum yield properties of a chimeric enzyme that contains the N-domain of Photinus pyralis luciferase joined to the C-domain of Luciola italica luciferase. Subsequently, we identified 5 amino acid changes based on L. italica that are the main determinants of the improved bioluminescence properties. Further engineering to enhance thermal and pH stability produced a novel luciferase called PLG2. We present here a systematic comparison of the spectral and physical properties of the new protein with P. pyralis luciferase and demonstrate the potential of PLG2 for use in assays based on the detection of femtomole levels of ATP. In addition, we compared the performance of a mammalian codon-optimized version of the cDNA for PLG2 with the luc2 gene in HEK293T cells. Using an optimized low-cost assay system, PLG2 activity can be monitored in mammalian cell lysates and living cells with 4.4-fold and approximately 3.0-fold greater sensitivity, respectively. PLG2 could be an improved alternative to Promega's luc2 for reporter and imaging applications.
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Registered trials
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