ArticleApplied and environmental microbiology2015
Physiology, Genomics, and Pathway Engineering of an Ethanol-Tolerant Strain of Clostridium phytofermentans.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 30 citations in OpenAlex.
- Review
- Tuning of Gene Expression inACS synthetic biology · 2022Article
- Article
- Developing Clostridia as Cell Factories for Short- and Medium-Chain Ester Production.Frontiers in bioengineering and biotechnology · 2021Review
- Artificial consortium demonstrates emergent properties of enhanced cellulosic-sugar degradation and biofuel synthesis.NPJ biofilms and microbiomes · 2020Article
- Metabolic and evolutionary responses ofBiotechnology for biofuels · 2020Article
- ABC Transporters Required for Hexose Uptake by Clostridium phytofermentans.Journal of bacteriology · 2019Article
- Amino acid catabolism-directed biofuel production inBiotechnology reports (Amsterdam, Netherlands) · 2017Review
- Evolution of a Biomass-Fermenting Bacterium To Resist Lignin Phenolics.Applied and environmental microbiology · 2017Article
- Determining the roles of the three alcohol dehydrogenases (AdhA, AdhB and AdhE) in Thermoanaerobacter ethanolicus during ethanol formation.Journal of industrial microbiology & biotechnology · 2017Article
- Metabolome analysis reveals a role for glyceraldehyde 3-phosphate dehydrogenase in the inhibition ofBiotechnology for biofuels · 2017Article
- Global repositioning of transcription start sites in a plant-fermenting bacterium.Nature communications · 2016Article
- Biofuels 2020: Biorefineries based on lignocellulosic materials.Microbial biotechnology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 4 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Novel processing strategies for hydrolysis and fermentation of lignocellulosic biomass in a single reactor offer large potential cost savings for production of biocommodities and biofuels. One critical challenge is retaining high enzyme production in the presence of elevated product titers. Toward this goal, the cellulolytic, ethanol-producing bacterium Clostridium phytofermentans was adapted to increased ethanol concentrations. The resulting ethanol-tolerant (ET) strain has nearly doubled ethanol tolerance relative to the wild-type level but also reduced ethanol yield and growth at low ethanol concentrations. The genome of the ET strain has coding changes in proteins involved in membrane biosynthesis, the Rnf complex, cation homeostasis, gene regulation, and ethanol production. In particular, purification of the mutant bifunctional acetaldehyde coenzyme A (CoA)/alcohol dehydrogenase showed that a G609D variant abolished its activities, including ethanol formation. Heterologous expression of Zymomonas mobilis pyruvate decarboxylase and alcohol dehydrogenase in the ET strain increased cellulose consumption and restored ethanol production, demonstrating how metabolic engineering can be used to overcome disadvantageous mutations incurred during adaptation to ethanol. We discuss how genetic changes in the ET strain reveal novel potential strategies for improving microbial solvent tolerance.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.