ArticleApplied and environmental microbiology2015
Implications of Genome-Based Discrimination between Clostridium botulinum Group I and Clostridium sporogenes Strains for Bacterial Taxonomy.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers, 1 of them a synthesis that pooled it.
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Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 59 citations in OpenAlex.
- Guideline
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- Real-time PCR assays that detect genes for botulinum neurotoxin A-G subtypes.Frontiers in microbiology · 2024Article
- Review
- Unintended consequences: Renaming botulinum neurotoxin-producing species of clostridium and related species.Toxicon : official journal of the International Society on Toxinology · 2023Review
- Potential Risk of Botulinum Neurotoxin -producing Clostridia Occurrence in Canned Fish.Journal of veterinary research · 2022Article
- Specific Isolation ofInternational journal of molecular sciences · 2022Article
- Selection and Development of Nontoxic NonproteolyticFoods (Basel, Switzerland) · 2022Article
- Genomic comparisons confirmFrontiers in cellular and infection microbiology · 2022Article
- Evaluation and Optimization of Microdrop Digital PCR for Detection of Serotype A and BFrontiers in microbiology · 2022Article
- Novel Lytic Enzyme of Prophage Origin fromInternational journal of molecular sciences · 2021Article
- Integration of Complete Plasmids ContainingToxins · 2021Article
- Investigation of the Effectiveness of Disinfectants Used in Meat-Processing Facilities to ControlFoods (Basel, Switzerland) · 2021Article
- The Distinctive Evolution ofFrontiers in microbiology · 2021Article
- Endogenous CRISPR-Cas Systems in Group IFrontiers in microbiology · 2021Article
- Whole Genome Sequence-Based Identification ofFrontiers in microbiology · 2021Article
- Efficient Secretion of Murine IL-2 From an Attenuated Strain ofFrontiers in microbiology · 2021Article
- Article
- Phage lysin that specifically eliminates Clostridium botulinum Group I cells.Scientific reports · 2020Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Taxonomic classification of Clostridium botulinum is based on the production of botulinum neurotoxin (BoNT), while closely related, nontoxic organisms are classified as Clostridium sporogenes. However, this taxonomic organization does not accurately mirror phylogenetic relationships between these species. A phylogenetic reconstruction using 2,016 orthologous genes shared among strains of C. botulinum group I and C. sporogenes clearly separated these two species into discrete clades which showed ∼93% average nucleotide identity (ANI) between them. Clustering of strains based on the presence of variable orthologs revealed 143 C. sporogenes clade-specific genetic signatures, a subset of which were further evaluated for their ability to correctly classify a panel of presumptive C. sporogenes strains by PCR. Genome sequencing of several C. sporogenes strains lacking these signatures confirmed that they clustered with C. botulinum strains in a core genome phylogenetic tree. Our analysis also identified C. botulinum strains that contained C. sporogenes clade-specific signatures and phylogenetically clustered with C. sporogenes strains. The genome sequences of two bont/B2-containing strains belonging to the C. sporogenes clade contained regions with similarity to a bont-bearing plasmid (pCLD), while two different strains belonging to the C. botulinum clade carried bont/B2 on the chromosome. These results indicate that bont/B2 was likely acquired by C. sporogenes strains through horizontal gene transfer. The genome-based classification of these species used to identify candidate genes for the development of rapid assays for molecular identification may be applicable to additional bacterial species that are challenging with respect to their classification.
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