ArticleLangmuir : the ACS journal of surfaces and colloids2015
Carboxybetaine Modified Interface for Electrochemical Glycoprofiling of Antibodies Isolated from Human Serum.
Article in Langmuir : the ACS journal of surfaces and colloids, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 32 citations in OpenAlex.
- Electrochemical Investigation of Interfacial Properties of TiFrontiers in chemistry · 2020Article
- Advanced impedimetric biosensor configuration and assay protocol for glycoprofiling of a prostate oncomarker using Au nanoshells with a magnetic core.Biosensors & bioelectronics · 2019Article
- Prostate-specific antigen glycoprofiling as diagnostic and prognostic biomarker of prostate cancer.Interface focus · 2019Review
- Label-free chronopotentiometric glycoprofiling of prostate specific antigen using sialic acid recognizing lectins.Bioelectrochemistry (Amsterdam, Netherlands) · 2017Article
- Nanotechnology in Glycomics: Applications in Diagnostics, Therapy, Imaging, and Separation Processes.Medicinal research reviews · 2017Review
- Recent Progress in Electrochemical Biosensors for Glycoproteins.Sensors (Basel, Switzerland) · 2016Review
- Electrochemical Impedance Spectroscopy Study of Concanavalin A Binding to Self-Assembled Monolayers of Mannosides on Gold Wire Electrodes.Journal of electroanalytical chemistry (Lausanne, Switzerland) · 2016Article
- Aberrant sialylation of a prostate-specific antigen: Electrochemical label-free glycoprofiling in prostate cancer serum samples.Analytica chimica acta · 2016Article
- Graphene oxide-based electrochemical label-free detection of glycoproteins down to aM level using a lectin biosensor.The Analyst · 2016Article
- Mixed Zwitterion-Based Self-Assembled Monolayer Interface for Impedimetric Glycomic Analyses of Human IgG Samples in an Array Format.Langmuir : the ACS journal of surfaces and colloids · 2016Article
- Glycan and lectin biosensors.Essays in biochemistry · 2016Review
- Ultrasensitive detection of influenza viruses with a glycan-based impedimetric biosensor.Biosensors & bioelectronics · 2016Article
- Sensitive detection and glycoprofiling of a prostate specific antigen using impedimetric assays.The Analyst · 2016Article
Corrections and comments
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Authors and funding
14 authors at 7 institutions in 3 countries.
Funding
Abstract
Impedimetric lectin biosensors capable of recognizing two different carbohydrates (galactose and sialic acid) in glycans attached to antibodies isolated from human serum were prepared. The first step entailed the modification of a gold surface by a self-assembled monolayer (SAM) deposited from a solution containing a carboxybetaine-terminated thiol applied to the subsequent covalent immobilization of lectins and to resist nonspecific protein adsorption. In the next step, Sambucus nigra agglutinin (SNA) or Ricinus communis agglutinin (RCA) was covalently attached to the SAM, and the whole process of building a bioreceptive layer was optimized and characterized using a diverse range of techniques including electrochemical impedance spectroscopy, cyclic voltammetry, quartz crystal microbalance, contact angle measurements, zeta-potential assays, X-ray photoelectron spectroscopy, and atomic force microscopy. In addition, the application of the SNA-based lectin biosensor in the glycoprofiling of antibodies isolated from the human sera of healthy individuals and of patients suffering from rheumatoid arthritis (RA) was successfully validated using an SNA-based lectin microarray. The results showed that the SNA lectin, in particular, is capable of discriminating between the antibodies isolated from healthy individuals and those from RA patients based on changes in the amount of sialic acid present in the antibodies. In addition, the results obtained by the application of RCA and SNA biosensors indicate that the abundance of galactose and sialic acid in antibodies isolated from healthy individuals is age-related.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.