Evidence map›Paper›PMID 26048030›Full record

ArticleClinical oral investigations2016

Expression patterns of immune genes in long-term cultured dental stem cells.

Pukana Jayaraman, Vijayendran Govindasamy, Nareshwaran Gnanasegaran, Wijenthiran Kunasekaran, Punitha Vasanthan, Sabri Musa, Noor Hayaty Abu Kasim

Abstract read
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In one paragraph

Article in Clinical oral investigations, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
1.2field-weighted citation impact, top 22% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 16 citations in OpenAlex.

  1. Article
  2. Article
  3. 1,25(OH)Journal of clinical medicine · 2019
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 1 institution in 1 country.

Pukana JayaramanGMP compliant Stem Cells Laboratory, Hygieia Innovation Sdn. Bhd, Federal Territory of Putrajaya, Lot 1G-2G, Lanai Complex, No.2, Persiaran Seri Perdana, Precinct 10, 62250, Putrajaya, Malaysia.
Vijayendran GovindasamyGMP compliant Stem Cells Laboratory, Hygieia Innovation Sdn. Bhd, Federal Territory of Putrajaya, Lot 1G-2G, Lanai Complex, No.2, Persiaran Seri Perdana, Precinct 10, 62250, Putrajaya, Malaysia. vijay@hygieiainnovation.com.
Nareshwaran GnanasegaranGMP compliant Stem Cells Laboratory, Hygieia Innovation Sdn. Bhd, Federal Territory of Putrajaya, Lot 1G-2G, Lanai Complex, No.2, Persiaran Seri Perdana, Precinct 10, 62250, Putrajaya, Malaysia.
Wijenthiran KunasekaranGMP compliant Stem Cells Laboratory, Hygieia Innovation Sdn. Bhd, Federal Territory of Putrajaya, Lot 1G-2G, Lanai Complex, No.2, Persiaran Seri Perdana, Precinct 10, 62250, Putrajaya, Malaysia.
Punitha VasanthanGMP compliant Stem Cells Laboratory, Hygieia Innovation Sdn. Bhd, Federal Territory of Putrajaya, Lot 1G-2G, Lanai Complex, No.2, Persiaran Seri Perdana, Precinct 10, 62250, Putrajaya, Malaysia.
Sabri MusaDepartment of Paediatric Dentistry and Orthodontics, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia. sabrim@um.edu.my.
Noor Hayaty Abu KasimDepartment of Restorative Dentistry, Faculty of Dentistry, University of Malaya, 50603, Kuala Lumpur, Malaysia.
University of Malaya · MY

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

BACKGROUND AND

objectivesLong-term culture system is used to prevent the impediment of insufficient cells and is good for low starting materials such as dental pulp or periodontal ligament. In general, although cell viability and functionality are the most common aspects taken into consideration in culturing cells for a long term, they may not truly represent the biological state of the cells. Hence, we explored the behaviour of another important aspect which is the immune properties in long-term cultured cells.

methodsDental pulp stem cells from deciduous (SHED; n = 3) and permanent (DPSCs; n = 3) teeth as well as periodontal ligament stem cells (PDLSCs; n = 3) were cultured under identical culture condition. The immune properties of each cell lines were profiled at passage 2 [P2] and passage 9 [P9] as early and late passages, respectively. This was further validated at the protein level using the Luminex platform.

resultsA major shift of genes was noticed at P9 with SHED being the highest. SHED cultured at P9 displayed many genes representing pathogen recognition (P < 0.001), immune signalling (P < 0.001, pro-inflammatory (P < 0.001), anti-inflammatory (P < 0.001) and immune-related growth and stimulation factor (P < 0.001) as compared to DPSCs and PDLSCs. Surprisingly, SHED also expressed many cytotoxicity genes (P < 0.001).

conclusionsCommunally, instabilities of immune genes from our findings suggest that long-term cultured cells may not be feasible for transplantation purposes. CLINICAL RELEVANCE: A complete biological characterization covering all major aspects including immune properties should be made as prerequisite criteria prior to the use of long-term cultured stem cells in clinical settings.

Indexed as

Gene Expression ProfilingAdultApoptosisCell Culture TechniquesCell DifferentiationCell ProliferationCells, CulturedChildCytokinesDental PulpHumansPeriodontal LigamentSignal TransductionStem CellsTooth, DeciduousUp-RegulationCytokinesChemokineCytokinesGene expression profileGrowth factorsImmune propertiesRegenerative medicine

Identifiers

PMID26048030
OpenAlexW608503267

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.