ArticleEukaryotic cell2015
Deletion of the DNA Ligase IV Gene in Candida glabrata Significantly Increases Gene-Targeting Efficiency.
Article in Eukaryotic cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 17 citations in OpenAlex.
- Targeting calcineurin: a synergistic strategy with caspofungin to induce farnesol-mediated quorum sensing in Nakaseomyces glabrata-an inoculum-dependent tale.The Biochemical journal · 2025Article
- A systematic comparison of CRISPR-Cas9 allele editing in Candida auris demonstrates unreliable cassette integration and effective episomal plasmid-based editing.Scientific reports · 2025Article
- A comparative evaluation of CRISPR-Cas9 allele editing systems inbioRxiv : the preprint server for biology · 2025Article
- Changes in Ergosterol Biosynthesis Alter the Response to Cycloheximide, 4-Nitroquinoline-N-Oxide, Weak Organic Acids, and Virulence inJournal of fungi (Basel, Switzerland) · 2024Article
- Enhancement of ROS Production by Catechin Is a Primary Effect of Increased Azole Efficacy inPathogens (Basel, Switzerland) · 2024Article
- Catechin potentiates the antifungal effect of miconazole in Candida glabrata.Folia microbiologica · 2023Article
- Essential Role ofJournal of fungi (Basel, Switzerland) · 2023Article
- Disruption of theJournal of fungi (Basel, Switzerland) · 2023Article
- Epigenetic Regulation of Antifungal Drug Resistance.Journal of fungi (Basel, Switzerland) · 2022Review
- The Gibberellin ProducerFrontiers in bioengineering and biotechnology · 2020Review
- Efficient gene targeting in non-homologous end-joining-deficient Lipomyces starkeyi strains.Current genetics · 2017Article
- Article
- Candida glabrata: new tools and technologies-expanding the toolkit.FEMS yeast research · 2015Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Candida glabrata is reported as the second most prevalent human opportunistic fungal pathogen in the United States. Over the last decades, its incidence increased, whereas that of Candida albicans decreased slightly. One of the main reasons for this shift is attributed to the inherent tolerance of C. glabrata toward the commonly used azole antifungal drugs. Despite a close phylogenetic distance to Saccharomyces cerevisiae, homologous recombination works with poor efficiency in C. glabrata compared to baker's yeast, in fact limiting targeted genetic alterations of the pathogen's genome. It has been shown that nonhomologous DNA end joining is dominant over specific gene targeting in C. glabrata. To improve the homologous recombination efficiency, we have generated a strain in which the LIG4 gene has been deleted, which resulted in a significant increase in correct gene targeting. The very specific function of Lig4 in mediating nonhomologous end joining is the reason for the absence of clear side effects, some of which affect the ku80 mutant, another mutant with reduced nonhomologous end joining. We also generated a LIG4 reintegration cassette. Our results show that the lig4 mutant strain may be a valuable tool for the C. glabrata research community.
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