ArticlePloS one2015
Fabrication of Biomimetic Bone Tissue Using Mesenchymal Stem Cell-Derived Three-Dimensional Constructs Incorporating Endothelial Cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Vascularization of a Bone Organoid Using Dental Pulp Stem Cells.Stem cells international · 2023Article
- Piezoelectric Signals in Vascularized Bone Regeneration.Biomolecules · 2021Review
- Article
- Injection of hybrid 3D spheroids composed of podocytes, mesenchymal stem cells, and vascular endothelial cells into the renal cortex improves kidney function and replenishes glomerular podocytes.Bioengineering & translational medicine · 2021Article
- Large three-dimensional cell constructs for tissue engineering.Science and technology of advanced materials · 2021Review
- Cross-Talk Between Mesenchymal Stromal Cells (MSCs) and Endothelial Progenitor Cells (EPCs) in Bone Regeneration.Frontiers in cell and developmental biology · 2021Review
- Effect of Integrin Binding Peptide on Vascularization of Scaffold-Free Microtissue Spheroids.Tissue engineering and regenerative medicine · 2020Article
- Bioactive factors for cartilage repair and regeneration: Improving delivery, retention, and activity.Acta biomaterialia · 2019Review
- Isolation and characterization of myogenic precursor cells from human cremaster muscle.Scientific reports · 2019Article
- 3D human bone marrow stromal and endothelial cell spheres promote bone healing in an osteogenic niche.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2019Article
- Bioprinting with human stem cell-laden alginate-gelatin bioink and bioactive glass for tissue engineering.International journal of bioprinting · 2019Article
- Temporal Transition of Mechanical Characteristics of HUVEC/MSC Spheroids Using a Microfluidic Chip with Force Sensor Probes.Micromachines · 2016Article
- Scaffold-Free Fabrication of Osteoinductive Cellular Constructs Using Mouse Gingiva-Derived Induced Pluripotent Stem Cells.Stem cells international · 2016Article
- Priming the Surface of Orthopedic Implants for Osteoblast Attachment in Bone Tissue Engineering.International journal of medical sciences · 2015Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of technologies to promote vascularization of engineered tissue would drive major developments in tissue engineering and regenerative medicine. Recently, we succeeded in fabricating three-dimensional (3D) cell constructs composed of mesenchymal stem cells (MSCs). However, the majority of cells within the constructs underwent necrosis due to a lack of nutrients and oxygen. We hypothesized that incorporation of vascular endothelial cells would improve the cell survival rate and aid in the fabrication of biomimetic bone tissues in vitro. The purpose of this study was to assess the impact of endothelial cells combined with the MSC constructs (MSC/HUVEC constructs) during short- and long-term culture. When human umbilical vein endothelial cells (HUVECs) were incorporated into the cell constructs, cell viability and growth factor production were increased after 7 days. Furthermore, HUVECs were observed to proliferate and self-organize into reticulate porous structures by interacting with the MSCs. After long-term culture, MSC/HUVEC constructs formed abundant mineralized matrices compared with those composed of MSCs alone. Transmission electron microscopy and qualitative analysis revealed that the mineralized matrices comprised porous cancellous bone-like tissues. These results demonstrate that highly biomimetic bone tissue can be fabricated in vitro by 3D MSC constructs incorporated with HUVECs.
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