Evidence map›Paper›PMID 26047102›Full record

ArticlePloS one2015

De Novo Assembly of Bitter Gourd Transcriptomes: Gene Expression and Sequence Variations in Gynoecious and Monoecious Lines.

Anjali Shukla, V K Singh, D R Bharadwaj, Rajesh Kumar, Ashutosh Rai, A K Rai, Raja Mugasimangalam, Sriram Parameswaran, Major Singh, P S Naik

Abstract read
In one paragraph

Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Anjali ShuklaDivision of Crop Improvement, Indian Institute of Vegetable Research, Post Bag No- 01, Jakhini (Shahanshahpur), Varanasi, Uttar Pradesh-221 305, India.
V K SinghCentre for Bioinformatics, School of Biotechnology, Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India.
D R BharadwajDivision of Crop Improvement, Indian Institute of Vegetable Research, Post Bag No- 01, Jakhini (Shahanshahpur), Varanasi, Uttar Pradesh-221 305, India.
Rajesh KumarDivision of Crop Improvement, Indian Institute of Vegetable Research, Post Bag No- 01, Jakhini (Shahanshahpur), Varanasi, Uttar Pradesh-221 305, India.
Ashutosh RaiDivision of Crop Improvement, Indian Institute of Vegetable Research, Post Bag No- 01, Jakhini (Shahanshahpur), Varanasi, Uttar Pradesh-221 305, India.
A K RaiCentre of Advanced Studies, Department of Botany, Banaras Hindu University, Varanasi, Uttar Pradesh 221 005, India.
Raja MugasimangalamGenotypic Technology (P) Ltd., Bangalore 560094, Karnataka, India.
Sriram ParameswaranGenotypic Technology (P) Ltd., Bangalore 560094, Karnataka, India.
Major SinghDivision of Crop Improvement, Indian Institute of Vegetable Research, Post Bag No- 01, Jakhini (Shahanshahpur), Varanasi, Uttar Pradesh-221 305, India.
P S NaikDivision of Crop Improvement, Indian Institute of Vegetable Research, Post Bag No- 01, Jakhini (Shahanshahpur), Varanasi, Uttar Pradesh-221 305, India.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Bitter gourd (Momordica charantia L.) is a nutritious vegetable crop of Asian origin, used as a medicinal herb in Indian and Chinese traditional medicine. Molecular breeding in bitter gourd is in its infancy, due to limited molecular resources, particularly on functional markers for traits such as gynoecy. We performed de novo transcriptome sequencing of bitter gourd using Illumina next-generation sequencer, from root, flower buds, stem and leaf samples of gynoecious line (Gy323) and a monoecious line (DRAR1). A total of 65,540 transcripts for Gy323 and 61,490 for DRAR1 were obtained. Comparisons revealed SNP and SSR variations between these lines and, identification of gene classes. Based on available transcripts we identified 80 WRKY transcription factors, several reported in responses to biotic and abiotic stresses; 56 ARF genes which play a pivotal role in auxin-regulated gene expression and development. The data presented will be useful in both functions studies and breeding programs in bitter gourd.

Indexed as

Genetic VariationTranscriptomeGene Expression Regulation, PlantGenes, PlantHigh-Throughput Nucleotide SequencingMedicine, TraditionalMicrosatellite RepeatsMomordica charantiaPlant ProteinsPolymorphism, Single NucleotideReal-Time Polymerase Chain ReactionSequence Analysis, RNATranscription FactorsPlant ProteinsTranscription Factors

Identifiers

PMID26047102
PMCPMC4457790

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.