ArticlePloS one2015
Novel and Stress Relevant EST Derived SSR Markers Developed and Validated in Peanut.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- Generation and characterization of novel transcriptome-derived SSR markers for genetic applications in blueberry.Scientific reports · 2025Article
- De Novo Mining and Validating Novel Microsatellite Markers to Assess Genetic Diversity inGenes · 2023Article
- Genome-wide characterization leading to simple sequence repeat (SSR) markers development in Shorea robusta.Functional & integrative genomics · 2023Article
- Comparative transcriptome analysis, unfolding the pathways regulating the seed-size trait in cultivated lentil (Frontiers in genetics · 2022Article
- Genic microsatellite marker characterization and development in little millet (Panicum sumatrense) using transcriptome sequencing.Scientific reports · 2021Article
- Article
- Identification of novel QTLs for late leaf spot resistance and validation of a major rust QTL in peanut (3 Biotech · 2020Article
- Article
- Characteristics of Microsatellites Mined from Transcriptome Data and the Development of Novel Markers inGenes · 2020Article
- Article
- Advances in the development and use ofPhysiology and molecular biology of plants : an international journal of functional plant biology · 2019Review
- Article
- Transcriptomic resources for the medicinal legume Mucuna pruriens: de novo transcriptome assembly, annotation, identification and validation of EST-SSR markers.BMC genomics · 2017Article
- De novo sequencing and comparative analysis of leaf transcriptomes of diverse condensed tannin-containing lines of underutilized Psophocarpus tetragonolobus (L.) DC.Scientific reports · 2017Article
- Biotechnological Advancements and Begomovirus Management in Okra (Frontiers in plant science · 2017Review
- Transcriptome sequencing and marker development in winged bean (Psophocarpus tetragonolobus; Leguminosae).Scientific reports · 2016Article
- Article
- Stress Inducible Expression of AtDREB1A Transcription Factor in Transgenic Peanut (Arachis hypogaea L.) Conferred Tolerance to Soil-Moisture Deficit Stress.Frontiers in plant science · 2016Article
- Characterization and Transferable Utility of Microsatellite Markers in the Wild and Cultivated Arachis Species.PloS one · 2016Article
- Correction: Novel and Stress Relevant EST Derived SSR Markers Developed and Validated in Peanut.PloS one · 2015Article
Corrections and comments
- Erratum issued
Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
With the aim to increase the number of functional markers in resource poor crop like cultivated peanut (Arachis hypogaea), large numbers of available expressed sequence tags (ESTs) in the public databases, were employed for the development of novel EST derived simple sequence repeat (SSR) markers. From 16424 unigenes, 2784 (16.95%) SSRs containing unigenes having 3373 SSR motifs were identified. Of these, 2027 (72.81%) sequences were annotated and 4124 gene ontology terms were assigned. Among different SSR motif-classes, tri-nucleotide repeats (33.86%) were the most abundant followed by di-nucleotide repeats (27.51%) while AG/CT (20.7%) and AAG/CTT (13.25%) were the most abundant repeat-motifs. A total of 2456 EST-SSR novel primer pairs were designed, of which 366 unigenes having relevance to various stresses and other functions, were PCR validated using a set of 11 diverse peanut genotypes. Of these, 340 (92.62%) primer pairs yielded clear and scorable PCR products and 39 (10.66%) primer pairs exhibited polymorphisms. Overall, the number of alleles per marker ranged from 1-12 with an average of 3.77 and the PIC ranged from 0.028 to 0.375 with an average of 0.325. The identified EST-SSRs not only enriched the existing molecular markers kitty, but would also facilitate the targeted research in marker-trait association for various stresses, inter-specific studies and genetic diversity analysis in peanut.
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