ArticlePloS one2015
Gastrointestinal Fibroblasts Have Specialized, Diverse Transcriptional Phenotypes: A Comprehensive Gene Expression Analysis of Human Fibroblasts.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed.
- Structured multicellular intestinal spheroids (SMIS) as a standardized model for infection biology.Gut pathogens · 2024Article
- Helicobacter pylori-activated fibroblasts as a silent partner in gastric cancer development.Cancer metastasis reviews · 2023Review
- Fibroblasts as Turned Agents in Cancer Progression.Cancers · 2023Review
- Functional genomics uncovers the transcription factor BNC2 as required for myofibroblastic activation in fibrosis.Nature communications · 2022Article
- Immunological Regulation of Intestinal Fibrosis in Inflammatory Bowel Disease.Inflammatory bowel diseases · 2022Review
- FibroDB: Expression Analysis of Protein-Coding and Long Non-Coding RNA Genes in Fibrosis.Non-coding RNA · 2022Article
- From the intestinal mucosal barrier to the enteric neuromuscular compartment: an integrated overview on the morphological changes in Parkinson's disease.European journal of histochemistry : EJH · 2021Review
- Organ Specificity and Heterogeneity of Cancer-Associated Fibroblasts in Colorectal Cancer.International journal of molecular sciences · 2021Review
- The bright side of fibroblasts: molecular signature and regenerative cues in major organs.NPJ Regenerative medicine · 2021Review
- Location, location, location: how the tissue microenvironment affects inflammation in RA.Nature reviews. Rheumatology · 2021Review
- Characterization of a bovine intestinal myofibroblast cell line and stimulation using phytoglycogen-based nanoparticles bound to inosine monophosphate.In vitro cellular & developmental biology. Animal · 2021Article
- Novel stromal biomarker screening in pancreatic cancer patients using the in vitro cancer-stromal interaction model.BMC gastroenterology · 2020Article
- Mechanism of fibrosis and stricture formation in Crohn's disease.Scandinavian journal of immunology · 2020Review
- Upregulation of cancer-associated gene expression in activated fibroblasts in a mouse model of non-alcoholic steatohepatitis.Scientific reports · 2019Article
- Tissue specific human fibroblast differential expression based on RNAsequencing analysis.BMC genomics · 2019Article
- Host tissue determinants of tumour immunity.Nature reviews. Cancer · 2019Review
- Batch adjustment by reference alignment (BARA): Improved prediction performance in biological test sets with batch effects.PloS one · 2019Article
- Differentiation of Human Pluripotent Stem Cells into Colonic Organoids via Transient Activation of BMP Signaling.Cell stem cell · 2017Article
- Tumor-associated fibroblasts predominantly come from local and not circulating precursors.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Distinct miRNA profiles in normal and gastric cancer myofibroblasts and significance in Wnt signaling.American journal of physiology. Gastrointestinal and liver physiology · 2016Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundFibroblasts are the principal stromal cells that exist in whole organs and play vital roles in many biological processes. Although the functional diversity of fibroblasts has been estimated, a comprehensive analysis of fibroblasts from the whole body has not been performed and their transcriptional diversity has not been sufficiently explored. The aim of this study was to elucidate the transcriptional diversity of human fibroblasts within the whole body.
methodsGlobal gene expression analysis was performed on 63 human primary fibroblasts from 13 organs. Of these, 32 fibroblasts from gastrointestinal organs (gastrointestinal fibroblasts: GIFs) were obtained from a pair of 2 anatomical sites: the submucosal layer (submucosal fibroblasts: SMFs) and the subperitoneal layer (subperitoneal fibroblasts: SPFs). Using hierarchical clustering analysis, we elucidated identifiable subgroups of fibroblasts and analyzed the transcriptional character of each subgroup.
resultsIn unsupervised clustering, 2 major clusters that separate GIFs and non-GIFs were observed. Organ- and anatomical site-dependent clusters within GIFs were also observed. The signature genes that discriminated GIFs from non-GIFs, SMFs from SPFs, and the fibroblasts of one organ from another organ consisted of genes associated with transcriptional regulation, signaling ligands, and extracellular matrix remodeling.
conclusionsGIFs are characteristic fibroblasts with specific gene expressions from transcriptional regulation, signaling ligands, and extracellular matrix remodeling related genes. In addition, the anatomical site- and organ-dependent diversity of GIFs was also discovered. These features of GIFs contribute to their specific physiological function and homeostatic maintenance, and create a functional diversity of the gastrointestinal tract.
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