ArticlePloS one2015
An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed.
- Extremophilic bacteriorhodopsin from hypersaline salt pan: characterization and photoelectrochemical assessment for potential biosensor applications.Frontiers in microbiology · 2026Article
- Ion-conducting and gating molecular mechanisms of channelrhodopsin revealed by true-atomic-resolution structures of open and closed states.Nature structural & molecular biology · 2025Article
- Overcoming Protein Orientation Mismatch Enables Efficient Nanoscale Light-Driven ATP Production.ACS synthetic biology · 2024Article
- Crystallographic insights into lipid-membrane protein interactions in microbial rhodopsins.Frontiers in molecular biosciences · 2024Article
- Article
- Expression of Xanthorhodopsin in Escherichia coli.The protein journal · 2023Article
- High-pressure crystallography shows noble gas intervention into protein-lipid interaction and suggests a model for anaesthetic action.Communications biology · 2022Article
- E. coli Expression and Purification of Microbial and Viral Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Rhodopsins: An Excitingly Versatile Protein Species for Research, Development and Creative Engineering.Frontiers in chemistry · 2022Review
- Molecular Biology of Microbial Rhodopsins.Methods in molecular biology (Clifton, N.J.) · 2022Review
- Molecular model of a sensor of two-component signaling system.Scientific reports · 2021Article
- Isolation and Taxonomic Characterization of Novel Haloarchaeal Isolates From Indian Solar Saltern: A Brief Review on Distribution of Bacteriorhodopsins and V-Type ATPases in Haloarchaea.Frontiers in microbiology · 2020Article
- Unique structure and function of viral rhodopsins.Nature communications · 2019Article
- High-throughput stability screening for detergent-solubilized membrane proteins.Scientific reports · 2019Article
- Discovery of bacteriorhodopsins in Haloarchaeal species isolated from Indian solar salterns: deciphering the role of the N-terminal residues in protein folding and functional expression.Microbial biotechnology · 2019Article
- Structure and mechanisms of sodium-pumping KR2 rhodopsin.Science advances · 2019Article
- Soluble Production of Human Recombinant VEGF-A121 by Using SUMO Fusion Technology in Escherichia coli.Molecular biotechnology · 2018Article
- Improving heterologous membrane protein production in Escherichia coli by combining transcriptional tuning and codon usage algorithms.PloS one · 2017Article
Corrections and comments
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allows one to localize the underlying problem of poor expression of a membrane protein using bR as an example. Our approach is based on constructing chimeric proteins comprising parts of a protein of interest and complementary parts of a homologous protein demonstrating advantageous expression. This complementary protein approach allowed us to increase bR expression by two orders of magnitude through the introduction of two silent mutations into bR coding DNA. For the first time the high quality crystals of bR expressed in E. Coli were obtained using the produced protein. The crystals obtained with in meso nanovolume crystallization diffracted to 1.67 Å.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.