ArticlePLoS neglected tropical diseases2015
Characterization of Spirometra erinaceieuropaei Plerocercoid Cysteine Protease and Potential Application for Serodiagnosis of Sparganosis.
Article in PLoS neglected tropical diseases, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers, 1 of them a synthesis that pooled it.
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Who cites it
36 citing papers in PubMed, 1 synthesis or guideline pooled it, 69 citations in OpenAlex.
- The global prevalence of Spirometra parasites in snakes, frogs, dogs, and cats: A systematic review and meta-analysis.Veterinary medicine and science · 2022Pooled it
- Development of PCR, qPCR and LAMP methods for the detection ofFood and waterborne parasitology · 2025Article
- Molecular Characteristics of the Malate Dehydrogenase (MDH) Gene Family inInternational journal of molecular sciences · 2024Article
- Application of a recombinant novel trypsin from Trichinella spiralis for serodiagnosis of trichinellosis.Parasites & vectors · 2024Article
- A novel trypsin of Trichinella spiralis mediates larval invasion of gut epithelium via binding to PAR2 and activating ERK1/2 pathway.PLoS neglected tropical diseases · 2024Article
- Hand palm sparganosis: morphologically and genetically confirmedJournal of parasitic diseases : official organ of the Indian Society for Parasitology · 2023Article
- Molecular characterization of a novel serine proteinase from Trichinella spiralis and its participation in larval invasion of gut epithelium.PLoS neglected tropical diseases · 2023Article
- The serum IgG antibody level as a biomarker for clinical outcome in patients with cerebral sparganosis after treatment.Frontiers in immunology · 2023Article
- Molecular characterization and determination of the biochemical properties of cathepsin L of Trichinella spiralis.Veterinary research · 2022Article
- Epidemiology, Diagnosis, and Prevention of Sparganosis in Asia.Animals : an open access journal from MDPI · 2022Review
- Proteases secreted by Trichinella spiralis intestinal infective larvae damage the junctions of the intestinal epithelial cell monolayer and mediate larval invasion.Veterinary research · 2022Article
- Molecular characteristics of glutathione transferase gene family in a neglect medicalFrontiers in veterinary science · 2022Article
- Proteomic and Immunological Identification of Diagnostic Antigens from Spirometra erinaceieuropaei Plerocercoid.The Korean journal of parasitology · 2021Article
- Oral vaccination with recombinant Lactobacillus plantarum encoding Trichinella spiralis inorganic pyrophosphatase elicited a protective immunity in BALB/c mice.PLoS neglected tropical diseases · 2021Article
- Characterization of a novel cysteine protease in Trichinella spiralis and its role in larval intrusion, development and fecundity.Veterinary research · 2021Article
- Characterization of a Novel Glutamine Synthetase FromFrontiers in cell and developmental biology · 2021Article
- Molecular characterization of a Trichinella spiralis serine proteinase.Veterinary research · 2020Article
- Vaccination of Mice with a Novel Trypsin fromVaccines · 2020Article
- Characterization of a Trichinella spiralis aminopeptidase and its participation in invasion, development and fecundity.Veterinary research · 2020Article
- The cysteine protease ATG4B of Trichinella spiralis promotes larval invasion into the intestine of the host.Veterinary research · 2020Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSparganosis is a neglected but important food-borne parasitic zoonosis. Clinical diagnosis of sparganosis is difficult because there are no specific manifestations. ELISA using plerocercoid crude or excretory-secretory (ES) antigens has high sensitivity but has cross-reactions with other helminthiases. The aim of this study was to characterize Spirometra erinaceieuropaei cysteine protease (SeCP) and to evaluate its potential application for serodiagnosis of sparganosis. METHODOLOGY/PRINCIPAL
findingsThe full length SeCP gene was cloned, and recombinant SeCP (rSeCP) was expressed and purified. Western blotting showed that rSeCP was recognized by the serum of sparganum-infected mice, and anti-rSeCP serum recognized the native SeCP protein of plerocercoid crude or ES antigens. Expression of SeCP was observed at plerocercoid stages but not at the adult and egg stages. Immunolocalization identified SeCP in plerocercoid tegument and parenchymal tissue. The rSeCP had CP activity, and the optimum pH and temperature were 5.5 and 37°C, respectively. Enzymatic activity was significantly inhibited by E-64. rSeCP functions to degrade different proteins and the function was inhibited by anti-rSeCP serum and E-64. Immunization of mice with rSeCP induced Th2-predominant immune responses and anti-rSeCP antibodies had the potential capabilities to kill plerocercoids in an ADCC assay. The sensitivity of rSeCP-ELISA and ES antigen ELISA was 100% when performed on sera of patients with sparganosis. The specificity of rSeCP-ELISA and ES antigen ELISA was 98.22% (166/169) and 87.57% (148/169), respectively (P<0.05).
conclusionsThe rSeCP had the CP enzymatic activity and SeCP seems to be important for the survival of plerocercoids in host. The rSeCP is a potential diagnostic antigen for sparganosis.
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