ArticleEpigenetics2015
Lead exposure induces changes in 5-hydroxymethylcytosine clusters in CpG islands in human embryonic stem cells and umbilical cord blood.
Article in Epigenetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 39 papers, 1 of them a synthesis that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
39 citing papers in PubMed, 1 synthesis or guideline pooled it, 66 citations in OpenAlex.
- 'Omics in environmental epidemiological studies of chemical exposures: A systematic evidence map.Environment international · 2022Pooled it
- Transcriptomic effects of lead on the differentiation of human embryonic stem cells into ectoderm: implication for adverse pregnancy outcomes and neurodevelopmental disorders.Archives of toxicology · 2026Article
- Environmental Toxicants in the Hispanic Community Epigenetically Contributing to Preeclampsia.Cardiovascular toxicology · 2025Review
- Review
- Pathological epigenetic events and reversibility review: the intersection between hallmarks of aging and developmental origin of health and disease.Epigenomics · 2023Review
- Sex differences in the intergenerational link between maternal and neonatal whole blood DNA methylation: a genome-wide analysis in 2 birth cohorts.Clinical epigenetics · 2023Article
- Developmental exposures to common environmental contaminants, DEHP and lead, alter adult brain and blood hydroxymethylation in mice.Frontiers in cell and developmental biology · 2023Article
- Prenatal lead (Pb) exposure is associated with differential placental DNA methylation and hydroxymethylation in a human population.Epigenetics · 2022Article
- Serum lead, mercury, manganese, and copper and DNA methylation age among adults in Detroit, Michigan.Environmental epigenetics · 2022Article
- Developmental toxicant exposures and sex-specific effects on epigenetic programming and cardiovascular health across generations.Environmental epigenetics · 2022Review
- 10 Years of Toxicogenomics section in Frontiers in Genetics: Past discoveries and Future Perspectives.Frontiers in genetics · 2022Review
- Tissue and sex-specific programming of DNA methylation by perinatal lead exposure: implications for environmental epigenetics studies.Epigenetics · 2021Article
- Review
- Prenatal Lead (Pb) Exposure and Peripheral Blood DNA Methylation (5mC) and Hydroxymethylation (5hmC) in Mexican Adolescents from the ELEMENT Birth Cohort.Environmental health perspectives · 2021Article
- Sex-Specific Alterations in Cardiac DNA Methylation in Adult Mice by Perinatal Lead Exposure.International journal of environmental research and public health · 2021Article
- DNA methylation changes induced by prenatal toxic metal exposure: An overview of epidemiological evidence.Environmental epigenetics · 2021Review
- Association Between miR-148a and DNA Methylation Profile in Individuals Exposed to Lead (Pb).Frontiers in genetics · 2021Article
- The Molecular Mechanisms of Adaptive Response Related to Environmental Stress.International journal of molecular sciences · 2020Review
- Neonatal Lead (Pb) Exposure and DNA Methylation Profiles in Dried Bloodspots.International journal of environmental research and public health · 2020Article
- Epigenetic Basis of Lead-Induced Neurological Disorders.International journal of environmental research and public health · 2020Review
Corrections and comments
- Erratum issuedCorrigendum.2015
Authors and funding
9 authors at 5 institutions in 5 countries.
Funding
Abstract
Prenatal exposure to neurotoxicants such as lead (Pb) may cause stable changes in the DNA methylation (5mC) profile of the fetal genome. However, few studies have examined its effect on the DNA de-methylation pathway, specifically the dynamic changes of the 5-hydroxymethylcytosine (5hmC) profile. Therefore, in this study, we investigate the relationship between Pb exposure and 5mC and 5hmC modifications during early development. To study the changes in the 5hmC profile, we use a novel modification of the Infinium™ HumanMethylation450 assay (Illumina, Inc.), which we named HMeDIP-450K assay, in an in vitro human embryonic stem cell model of Pb exposure. We model Pb exposure-associated 5hmC changes as clusters of correlated, adjacent CpG sites, which are co-responding to Pb. We further extend our study to look at Pb-dependent changes in high density 5hmC regions in umbilical cord blood DNA from 48 mother-infant pairs from the Early Life Exposure in Mexico to Environmental Toxicants (ELEMENT) cohort. For our study, we randomly selected umbilical cord blood from 24 male and 24 female children from the 1st and 4th quartiles of Pb levels. Our data show that Pb-associated changes in the 5hmC and 5mC profiles can be divided into sex-dependent and sex-independent categories. Interestingly, differential 5mC sites are better markers of Pb-associated sex-dependent changes compared to differential 5hmC sites. In this study we identified several 5hmC and 5mC genomic loci, which we believe might have some potential as early biomarkers of prenatal Pb exposure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.