Evidence map›Paper›PMID 26046668›Full record

ArticleScientific reports2015

Printed Flexible Plastic Microchip for Viral Load Measurement through Quantitative Detection of Viruses in Plasma and Saliva.

Hadi Shafiee, Manoj Kumar Kanakasabapathy, Franceline Juillard, Mert Keser, Magesh Sadasivam, Mehmet Yuksekkaya, Emily Hanhauser, Timothy J Henrich, Daniel R Kuritzkes, Kenneth M Kaye and 1 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
3.4field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 29 citations in OpenAlex.

  1. Review
  2. The emerging role of cancer nanotechnology in the panorama of sarcoma.Frontiers in bioengineering and biotechnology · 2022
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 3 institutions in 1 country.

Hadi Shafiee1] Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA.
Manoj Kumar Kanakasabapathy1] Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA.
Franceline JuillardDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Mert Keser1] Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA.
Magesh Sadasivam1] Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA.
Mehmet Yuksekkaya1] Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Harvard-MIT Division of Health Sciences and Technology, Cambridge, MA, USA.
Emily HanhauserDivision of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, MA, USA.
Timothy J HenrichDivision of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, MA, USA.
Daniel R KuritzkesDivision of Infectious Diseases, Brigham and Women's Hospital, Harvard Medical School, MA, USA.
Kenneth M KayeDepartment of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA.
Utkan Demirci1] Division of Biomedical Engineering, Division of Renal Medicine, Department of Medicine, Brigham and Women's Hospital, Harvard Medical School, Boston, MA, USA [2] Department of Radiology, Canary Center at Stanford for Cancer Early Detection, Stanford University School of Medicine, Palo Alto, CA, USA.
Brigham and Women's Hospital · USHarvard University · USStanford University · US

Funding

Rapid Fluorescence-Based Determination of Antibiotic SusceptibilityU54EB015408 · NIBIB · MASSACHUSETTS GENERAL HOSPITAL · PI PARRISH, JOHN A, SCHACHTER, STEVEN · 2012 to 2022
$49.2M
GENETIC AND BIOCHEMICAL STUDIES OF THE KSHV LANA GENER01CA082036 · NCI · BRIGHAM AND WOMEN'S HOSPITAL · PI KAYE, KENNETH M · 1999 to 2019
$7.6M
Point-of-Care Rapid Blood Testing Using Microfluidic DevicesR01AI081534 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI DEMIRCI, UTKAN · 2009 to 2013
$2.1M
Photonic Crystal Surfaces for Label-Free Detection and Fluorescence AmplificationR01GM086382 · NIGMS · UNIVERSITY OF ILLINOIS AT URBANA-CHAMPAIGN · PI BOLLERO, GERMAN A, CUNNINGHAM, BRIAN T. · 2009 to 2012
$1.7M
Novel disposable microchips for HIV-1 viral loadR01AI093282 · NIAID · STANFORD UNIVERSITY · PI DEMIRCI, UTKAN · 2012 to 2015
$1.7M
Antibody-based cell purification at resource limited setting for HIVR21AI087107 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI DEMIRCI, UTKAN · 2010 to 2011
$488k
Label-free HIV-1 Viral Load Measurement through a Contactless Electrode MicrochipF32AI102590 · NIAID · BRIGHAM AND WOMEN'S HOSPITAL · PI SHAFIEE, HADI · 2013 to 2015
$171k
NCI NIH HHS R01 CA082036NCI NIH HHS R01CA082036NIAID NIH HHS F32 AI102590NIAID NIH HHS F32AI102590NIAID NIH HHS R01 AI081534NIAID NIH HHS R01AI081534NIAID NIH HHS R01 AI093282NIAID NIH HHS R01AI093282NIAID NIH HHS R21 AI087107NIAID NIH HHS R21AI087107NIBIB NIH HHS U54 EB015408NIBIB NIH HHS U54EB15408NIGMS NIH HHS R01 GM086382NIGMS NIH HHS R01GM086382
6 · The paper itself

Abstract

We report a biosensing platform for viral load measurement through electrical sensing of viruses on a flexible plastic microchip with printed electrodes. Point-of-care (POC) viral load measurement is of paramount importance with significant impact on a broad range of applications, including infectious disease diagnostics and treatment monitoring specifically in resource-constrained settings. Here, we present a broadly applicable and inexpensive biosensing technology for accurate quantification of bioagents, including viruses in biological samples, such as plasma and artificial saliva, at clinically relevant concentrations. Our microchip fabrication is simple and mass-producible as we print microelectrodes on flexible plastic substrates using conductive inks. We evaluated the microchip technology by detecting and quantifying multiple Human Immunodeficiency Virus (HIV) subtypes (A, B, C, D, E, G, and panel), Epstein-Barr Virus (EBV), and Kaposi's Sarcoma-associated Herpes Virus (KSHV) in a fingerprick volume (50 µL) of PBS, plasma, and artificial saliva samples for a broad range of virus concentrations between 10(2) copies/mL and 10(7) copies/mL. We have also evaluated the microchip platform with discarded, de-identified HIV-infected patient samples by comparing our microchip viral load measurement results with reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) as the gold standard method using Bland-Altman Analysis.

Indexed as

Viral LoadEpstein-Barr Virus InfectionsHerpesviridae InfectionsHerpesvirus 4, HumanHerpesvirus 8, HumanHIV-1HIV InfectionsHumansLab-On-A-Chip DevicesPoint-of-Care SystemsReference Standards

Identifiers

PMID26046668
PMCPMC4456945
OpenAlexW1859924640

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.