ArticleCell2015
The Transcriptome and DNA Methylome Landscapes of Human Primordial Germ Cells.
Open access · bronzeAbstract read
In one paragraph
Article in Cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 325 papers.
0numbers the graph read from it
0cells of the map it votes in
325citing papers in PubMed
27.9field-weighted citation impact, top 1% of its field
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
325 citing papers in PubMed, 625 citations in OpenAlex.
- TRIM37 interacts with TRIM28 to maintain primordial germ cell identity during migration.Cell research · 2026Article
- Characterization of the metabolome and proteome of stem cell-derived human primordial germ cell-like cells: a multi-omics approach.Biology open · 2026Article
- Mapping ovarian cellular and molecular landscape across the lifespan of women: a scoping review.Human reproduction update · 2026Article
- Article
- Generation of human primordial germ cell-like cells from cumulus and blood cell-derived isogenic human induced pluripotent stem cells†.Biology of reproduction · 2026Article
- Epigenetic blind spots - the role of DNA methylation dynamics in stem cell-based models of embryogenesis.FEBS letters · 2026Article
- High-sensitive glycomics using seGRAP-mass spectrometry uncovers a conserved N-glycome in single human oocytes.Nature communications · 2026Article
- Human primordial germ cell heterogeneity in vitro is associated with distinctive biological states.Cellular & molecular biology letters · 2026Article
- Lineage tracing of soma-to-primordial germ cell-like conversion in human tumor cell line.iScience · 2026Article
- Developmental Morphokinetics and the Transcriptomic Profile of Bovine First-Cleaved Embryos: Normal vs. Abnormal Divisions.International journal of molecular sciences · 2026Article
- The multi-omics regulatory landscape of human early embryogenesis.Cellular and molecular life sciences : CMLS · 2026Review
- The interferon-stimulated gene product HERC5 inhibits human LINE-1 retrotransposition with an ISGylation-independent mechanism.Nucleic acids research · 2026Article
- A spatiotemporal transcriptomic atlas of porcine (Sus scrofa) female early gonadal development.Communications biology · 2026Article
- Reprogramming the inactive X chromosome: dynamics and insights from the germline.Biochemical Society transactions · 2026Review
- Reconstitution of the cellular niche requirements for primordial germ cell-like cell progression in humans.Stem cell reports · 2026Article
- Methylation profile characteristics in the H19/IGF2:IG-DMR revealed by long-read sequencing analysis in patients with Beckwith-Wiedemann syndrome having defects in the OCT4/SOX2 binding site.Clinical epigenetics · 2026Article
- Mechanisms of human germ cell development.Nature reviews. Molecular cell biology · 2026Review
- High resolution multi-scale profiling of embryonic germ cell-like cell derivation reveals pluripotent state transitions in humans.Stem cell reports · 2026Article
- Functional Validation of a Novel <italic>PBX1</italic> Missense Variant in a 46,XY Girl.Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation · 2026Article
- ELAV/Hu RNA-binding protein family: key regulators in neurological disorders, cancer, and other diseases.RNA biology · 2025Review
265 more citing papers are in PubMed but not listed here.
Corrections and comments
- Commented on by
Authors and funding
28 authors at 2 institutions in 4 countries.
Fan GuoBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC.
Liying YanBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Hongshan GuoBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC.
Lin LiBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC.
Boqiang HuBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC.
Yangyu ZhaoBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Jun YongBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC.
Yuqiong HuBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, PRC; Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100871, PRC.
Xiaoye WangBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Yuan WeiBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Wei WangBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Rong LiBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Jie YanBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Xu ZhiBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Yan ZhangBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Hongyan JinBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Wenxin ZhangBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Yu HouBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC.
Ping ZhuBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, PRC.
Jingyun LiBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, PRC.
Ling ZhangInstitute of Theoretical and Computational Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PRC.
Sirui LiuInstitute of Theoretical and Computational Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PRC.
Yixin RenBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Xiaohui ZhuBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC.
Lu WenBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, PRC.
Yi Qin GaoInstitute of Theoretical and Computational Chemistry, College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, PRC.
Fuchou TangBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Ministry of Education Key Laboratory of Cell Proliferation and Differentiation, Beijing 100871, PRC; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, PRC; Center for Molecular and Translational Medicine, Peking University Health Science Center, Beijing 100191, PRC. Electronic address: tangfuchou@pku.edu.cn.
Jie QiaoBiodynamic Optical Imaging Center and Department of Obstetrics and Gynecology, College of Life Sciences, Third Hospital, Peking University, Beijing 100871, PRC; Key Laboratory of Assisted Reproduction, Ministry of Education, Beijing 100191, PRC; Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, PRC; Beijing Key Laboratory of Reproductive Endocrinology and Assisted Reproduction, Beijing 100191, PRC. Electronic address: jie.qiao@263.net.
Ministry of Education of the People's Republic of China · CNPeking University · CN
Funding
No grant is acknowledged in the PubMed record.
Abstract
Germ cells are vital for transmitting genetic information from one generation to the next and for maintaining the continuation of species. Here, we analyze the transcriptome of human primordial germ cells (PGCs) from the migrating stage to the gonadal stage at single-cell and single-base resolutions. Human PGCs show unique transcription patterns involving the simultaneous expression of both pluripotency genes and germline-specific genes, with a subset of them displaying developmental-stage-specific features. Furthermore, we analyze the DNA methylome of human PGCs and find global demethylation of their genomes. Approximately 10 to 11 weeks after gestation, the PGCs are nearly devoid of any DNA methylation, with only 7.8% and 6.0% of the median methylation levels in male and female PGCs, respectively. Our work paves the way toward deciphering the complex epigenetic reprogramming of the germline with the aim of restoring totipotency in fertilized oocytes.
Indexed as
DNA MethylationTranscriptomeCell MovementChromosomes, Human, XCluster AnalysisEmbryo, MammalianFemaleGerm CellsHistonesHumansMalePrincipal Component AnalysisSOX Transcription FactorsHistonesSOX15 protein, humanSOX Transcription Factors
Identifiers
What OpenQuestion holds
Textmetadata
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.